Transmission of Raccoon-Passaged Chronic Wasting Disease Agent to White-Tailed Deer

Cassmann, Eric D.; Frese, Alexis J.; Moore, S. Jo; Greenlee, Justin J.

doi:10.3390/v14071578

Transmission of Raccoon-Passaged Chronic Wasting Disease Agent to White-Tailed Deer

Journal Article · Wed Jul 20 00:00:00 EDT 2022 · Viruses

DOI:https://doi.org/10.3390/v14071578· OSTI ID:1905062

^[1]; ^[2]; Moore, S. Jo ^[2]; ^[3]

US Dept. of Agriculture (USDA)., Ames, IA (United States); OSTI
US Dept. of Agriculture (USDA)., Ames, IA (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
US Dept. of Agriculture (USDA)., Ames, IA (United States)

The transmission characteristics of prion diseases are influenced by host prion protein sequence and, therefore, the host species. Chronic wasting disease (CWD), a prion disease of cervids, has widespread geographical distribution throughout North America and occurs in both wild and farmed populations. CWD prions contaminate the environment through scattered excrement and decomposing carcasses. Fresh carcasses with CWD prions are accessible by free-ranging mesopredators such as raccoons and may provide a route of exposure. Previous studies demonstrated the susceptibility of raccoons to CWD from white-tailed deer. In this study, we demonstrate that white-tailed deer replicate raccoon-passaged CWD prions which results in clinical disease similar to intraspecies CWD transmission. Six white-tailed deer were oronasally inoculated with brain homogenate from a raccoon with CWD. All six deer developed clinical disease, had widespread lymphoid distribution of misfolded CWD prions (PrP^Sc), and had neuropathologic lesions with PrP^Sc accumulation in the brain. The presence of PrP^Sc was confirmed by immunohistochemistry, enzyme-linked immunoassay, and western blot. The western blot migration pattern of raccoon-passaged CWD was different from white-tailed deer CWD. Transmission of raccoon CWD back to white-tailed deer resulted in an interposed molecular phenotype that was measurably different from white-tailed deer CWD.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: US Dept. of Agriculture (USDA); USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1905062

Journal Information:: Viruses, Journal Name: Viruses Journal Issue: 7 Vol. 14; ISSN 1999-4915

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
chronic wasting disease
interspecies transmission
prion
transmissible spongiform encephalopathy
virology
white-tailed deer

Transmission of Raccoon-Passaged Chronic Wasting Disease Agent to White-Tailed Deer

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