Oxidized Amino Acid Residues in the Vicinity of QA and PheoD1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species

Frankel, Laurie K.; Sallans, Larry; Limbach, Patrick A.; Bricker, Terry M.

doi:10.1371/journal.pone.0058042

Oxidized Amino Acid Residues in the Vicinity of Q_Aand Pheo_D1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species

Journal Article · Thu Feb 28 00:00:00 EST 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0058042· OSTI ID:1904572

Frankel, Laurie K. ^[1]; Sallans, Larry ^[2]; Limbach, Patrick A. ^[2]; Bricker, Terry M. ^[3]

Louisiana State University, Baton Rouge, LA (United States); Louisiana State Univ., Baton Rouge, LA (United States)
University of Cincinnati, OH (United States)
Louisiana State University, Baton Rouge, LA (United States)

Under a variety of stress conditions, Photosystem II produces reactive oxygen species on both the reducing and oxidizing sides of the photosystem. A number of different sites including the Mn₄O₅Ca cluster, P₆₈₀, Pheo_D1, Q_A, Q_B and cytochrome b559 have been hypothesized to produce reactive oxygen species in the photosystem. In this communication using Fourier transform ion cyclotron resonance mass spectrometry we have identified several residues on the D1 and D2 proteins from spinach which are oxidatively modified and in close proximity to Q_A(D1 residues ²³⁹F, ²⁴¹Q, ²⁴²E and the D2 residues ²³⁸P, ²³⁹T, ²⁴²E and ²⁴⁷M) and Pheo_D1 (D1 residues ¹³⁰E, ¹³³L and ¹³⁵F). These residues may be associated with reactive oxygen species exit pathways located on the reducing side of the photosystem, and their modification may indicate that both Q_A and Pheo_D1 are sources of reactive oxygen species on the reducing side of Photosystem II.

View Accepted Manuscript (DOE)

Research Organization:: Louisiana State University, Baton Rouge, LA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-98ER20310

OSTI ID:: 1904572

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 2 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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