Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II

Kumar, Aditya; Prasad, Ankush; Sedlářová, Michaela; Kale, Ravindra; Frankel, Laurie K.; Sallans, Larry; Bricker, Terry M.; Pospíšil, Pavel

doi:10.1073/pnas.2019246118

Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II

Journal Article · Thu Jan 21 00:00:00 EST 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.2019246118· OSTI ID:1850186

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Palacký Univ., Olomouc (Czech Republic). Faculty of Science. Centre of the Region Haná for Biotechnological and Agricultural Research. Dept. of Biophysics; Louisiana State Univ., Baton Rouge, LA (United States)
Palacký Univ., Olomouc (Czech Republic). Faculty of Science. Centre of the Region Haná for Biotechnological and Agricultural Research. Dept. of Biophysics
Palacký Univ., Olomouc (Czech Republic). Faculty of Science. Dept. of Botany
Louisiana State Univ., Baton Rouge, LA (United States). Division of Biochemistry and Molecular Biology. Dept. of Biological Sciences
Univ. of Cincinnati, OH (United States). Dept. of Chemistry. The Rieveschl Laboratories for Mass Spectrometry

Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study, oxidative modifications of the D1 and D2 proteins by the superoxide anion (O₂^•-) and the hydroxyl (HO•) radicals were studied in WT and a tocopherol cyclase (vte1) mutant, which is deficient in the lipid-soluble antioxidant α-tocopherol. In the absence of this antioxidant, high-resolution tandem mass spectrometry was used to identify oxidation of D1:¹³⁰E to hydroxyglutamic acid by O₂^•- at the Pheo_D1 site. Additionally, D1:²⁴⁶Y was modified to either tyrosine hydroperoxide or dihydroxyphenylalanine by O₂^•- and HO^•, respectively, in the vicinity of the nonheme iron. We propose that α-tocopherol is localized near PheoD1 and the nonheme iron, with its chromanol head exposed to the lipid–water interface. This helps to prevent oxidative modification of the amino acid’s hydrogen that is bonded to PheoD1 and the nonheme iron (via bicarbonate), and thus protects electron transport in PSII from ROS damage.

View Accepted Manuscript (DOE)

Research Organization:: Louisiana State Univ., Baton Rouge, LA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-98ER20310

OSTI ID:: 1850186

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 4 Vol. 118; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
EPR
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reactive oxygen species
science & technology
tocopherol

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