Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II

Kale, Ravindra; Hebert, Annette E.; Frankel, Laurie K.; Sallans, Larry; Bricker, Terry M.; Pospisil, Pavel

doi:10.1073/pnas.1618922114

Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II

Journal Article · Mon Mar 06 00:00:00 EST 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1618922114· OSTI ID:1465487

Kale, Ravindra ^[1]; Hebert, Annette E. ^[2]; Frankel, Laurie K. ^[2]; Sallans, Larry ^[3]; Bricker, Terry M. ^[2]; Pospisil, Pavel ^[4]

Palacky Univ., Olomouc (Czech Republic); Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, 783 71 Olomouc, Czech Republic
Louisiana State Univ., Baton Rouge, LA (United States)
Univ. of Cincinnati, Cincinnati, OH (United States)
Palacky Univ., Olomouc (Czech Republic)

The Photosystem II reaction center is vulnerable to photoinhibition. The D1 and D2 proteins, lying at the core of the photosystem, are susceptible to oxidative modification by reactive oxygen species that are formed by the photosystem during illumination. Using spin probes and EPR spectroscopy, we have determined that both O₂^•– and HO^• are involved in the photoinhibitory process. Using tandem mass spectroscopy, we have identified a number of oxidatively modified D1 and D2 residues. Our analysis indicates that these oxidative modifications are associated with formation of HO^• at both the Mn₄O₅Ca cluster and the nonheme iron. Additionally, O₂^•– appears to be formed by the reduction of O₂ at either Pheo_D1 or Q_A. Early oxidation of D1:³³²H, which is coordinated with the Mn1 of the Mn₄O₅Ca cluster, appears to initiate a cascade of oxidative events that lead to the oxidative modification of numerous residues in the C termini of the D1 and D2 proteins on the donor side of the photosystem. Oxidation of D2:²⁴⁴Y, which is a bicarbonate ligand for the nonheme iron, induces the propagation of oxidative reactions in residues of the D-de loop of the D2 protein on the electron acceptor side of the photosystem. Finally, D1:¹³⁰E and D2:²⁴⁶M are oxidatively modified by O₂^•– formed by the reduction of O₂ either by Pheo_D1^•– or Q_A^•–. Furthermore, the identification of specific amino acid residues oxidized by reactive oxygen species provides insights into the mechanism of damage to the D1 and D2 proteins under light stress.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Cincinnati, Cincinnati, OH (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-98ER20310

OSTI ID:: 1465487

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 11 Vol. 114; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Photosystem II
mass spectrometry
photo inhibition
photosynthesis
reactive oxygen species

Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II

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