Detecting the orientation of newly-deposited crystalline cellulose with fluorescent CBM3

Pfaff, Sarah A.; Wang, Xuan; Wagner, Edward R.; Wilson, Liza A.; Kiemle, Sarah N.; Cosgrove, Daniel J.

doi:10.1016/j.tcsw.2022.100089

Title: Detecting the orientation of newly-deposited crystalline cellulose with fluorescent CBM3

Journal Article · Thu Dec 01 00:00:00 EST 2022 · The Cell Surface

DOI:https://doi.org/10.1016/j.tcsw.2022.100089· OSTI ID:1899754

Pfaff, Sarah A.; Wang, Xuan; Wagner, Edward R.; Wilson, Liza A.; Kiemle, Sarah N.; Cosgrove, Daniel J.

Cellulose microfibril patterning influences many of the mechanical attributes of plant cell walls. We developed a simple, fluorescence microscopy-based method to detect the orientation of newly-synthesized cellulose microfibrils in epidermal peels of onion and Arabidopsis. It is based on Alexa Fluor 488-tagged carbohydrate binding module 3a (CBM3a) from Clostridium thermocellum which displayed a nearly 4-fold greater binding to cell walls at pH 5.5 compared with pH 8. Binding to isolated cellulose did not display this pH dependence. At pH 7.5 fibrillar patterns at the surface of the epidermal peels were visible, corresponding to the directionality of surface cellulose microfibrils, as verified by atomic force microscopy. The fibrillar pattern was not visible as the labeling intensity increased at lower pH. The pH of greatest cell wall labeling corresponds to the isoelectric point of CBM3a, suggesting that electrostatic forces limit CBM3a penetration into the wall. Consistent with this, digestion of the wall with pectate lyase to remove homogalacturonan increased labeling intensity. We conclude that electrostatic interactions strongly influence labeling of cell walls with CBM3 and potentially other proteins, holding implications for any work that relies on penetration of protein probes such as CBMs, antibodies, or enzymes into charged polymeric substrates.

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Research Organization:: Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1899754

Alternate ID(s):: OSTI ID: 2204037

Journal Information:: The Cell Surface, Journal Name: The Cell Surface Vol. 8 Journal Issue: C; ISSN 2468-2330

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Carbohydrate binding module 3a
Epidermal cell wall
Cellulose fibrils
Isoelectric point
Surface charge
Electrostatic interactions

Title: Detecting the orientation of newly-deposited crystalline cellulose with fluorescent CBM3

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