Transcriptional competition shapes proteotoxic ER stress resolution

Ko, Dae Kwan; Brandizzi, Federica

doi:10.1038/s41477-022-01150-w

Title: Transcriptional competition shapes proteotoxic ER stress resolution

Journal Article · Mon May 16 00:00:00 EDT 2022 · Nature Plants (Online)

DOI:https://doi.org/10.1038/s41477-022-01150-w· OSTI ID:1876165

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Michigan State Univ., East Lansing, MI (United States)

Through dynamic activities of conserved master transcription factors (mTFs), the unfolded protein response (UPR) relieves proteostasis imbalance of the endoplasmic reticulum (ER), a condition known as ER stress. Because dysregulated UPR is lethal, the competence for fate changes of the UPR mTFs must be tightly controlled. However, the molecular mechanisms underlying regulatory dynamics of mTFs remain largely elusive. Here, we identified the abscisic acid-related regulator G-class bZIP TF2 (GBF2) and the cis-regulatory element G-box as regulatory components of the plant UPR led by the mTFs, bZIP28 and bZIP60. We demonstrate that, by competing with the mTFs at G-box, GBF2 represses UPR gene expression. Conversely, a gbf2 null mutation enhances UPR gene expression and suppresses the lethality of a bzip28 bzip60 mutant in unresolved ER stress. Here, by demonstrating that GBF2 functions as a transcriptional repressor of the UPR, we address the long-standing challenge of identifying shared signalling components for a better understanding of the dynamic nature and complexity of stress biology. Furthermore, our results identify a new layer of UPR gene regulation hinged upon an antagonistic mTFs-GFB2 competition for proteostasis and cell fate determination.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH)

Contributing Organization:: University of California

Grant/Contract Number:: SC0018409; FG02-91ER20021

OSTI ID:: 1876165

Journal Information:: Nature Plants (Online), Vol. 8, Issue 5; ISSN 2055-0278

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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