Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

Zhang, Ningning; Pazouki, Leila; Nguyen, Huong; Jacobshagen, Sigrid; Bigge, Brae M.; Xia, Ming; Mattoon, Erin M.; Klebanovych, Anastasiya; Sorkin, Maria; Nusinow, Dmitri A.; Avasthi, Prachee; Czymmek, Kirk J.; Zhang, Ru

doi:10.3390/plants11050585

Title: Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

Journal Article · Tue Feb 22 00:00:00 EST 2022 · Plants

DOI:https://doi.org/10.3390/plants11050585· OSTI ID:1864761

Zhang, Ningning ^[1]; Pazouki, Leila ^[1]; Nguyen, Huong ^[1]; Jacobshagen, Sigrid ^[2];

^[3]; Xia, Ming ^[1]; Mattoon, Erin M. ^[4];

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Donald Danforth Plant Science Center, St. Louis, MO (United States)
Western Kentucky University, Bowling Green, KY (United States)
Dartmouth College, Hanover, NH (United States). Geisel School of Medicine
Donald Danforth Plant Science Center, St. Louis, MO (United States); Washington Univ., St. Louis, MO (United States)

The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly used background strains of Chlamydomonas are CC-1690 and CC-5325. CC-1690, also called 21gr, has been used for the Chlamydomonas genome project and several transcriptome analyses. CC-5325 is the background strain for the Chlamydomonas Library Project (CLiP). Photosynthetic performance in CC-5325 has not been evaluated in comparison with CC-1690. Additionally, CC-5325 is often considered to be cell-wall deficient, although detailed analysis is missing. The circadian rhythms in CC-5325 are also unclear. To fill these knowledge gaps and facilitate the use of the CLiP mutant library for various screens, we performed phenotypic comparisons between CC-1690 and CC-5325. Our results showed that CC-5325 grew faster heterotrophically in dark and equally well in mixotrophic liquid medium as compared to CC-1690. CC-5325 had lower photosynthetic efficiency and was more heat-sensitive than CC-1690. Furthermore, CC-5325 had an intact cell wall which had comparable integrity to that in CC-1690 but appeared to have reduced thickness. Additionally, CC-5325 could perform phototaxis, but could not maintain a sustained circadian rhythm of phototaxis as CC1690 did. Finally, in comparison to CC-1690, CC-5325 had longer cilia in the medium with acetate but slower swimming speed in the medium without nitrogen and acetate. Our results will be useful for researchers in the Chlamydomonas community to choose suitable background strains for mutant analysis and employ the CLiP mutant library for genome-wide mutant screens under appropriate conditions, especially in the areas of photosynthesis, thermotolerance, cell wall, and circadian rhythms.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS)

Grant/Contract Number:: SC0019464; SC0020400; R35GM128702

OSTI ID:: 1864761

Alternate ID(s):: OSTI ID: 2318490

Journal Information:: Plants, Vol. 11, Issue 5; ISSN 2223-7747

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Journal Article · Tue Aug 30 00:00:00 EDT 2022 · mSystems · OSTI ID:1864761

Metcalf, Alex J.; Boyle, Nanette R.; Bernstein, ed., Hans C.; +1 more

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59 BASIC BIOLOGICAL SCIENCES
Chlamydomonas reinhardtii
CC-1690
CC-5325
Chlamydomonas Library Project (CLiP)
photosynthesis
heat tolerance
cell wall
circadian rhythm of phototaxis

Title: Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

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