Phototaxis in a wild isolate of the cyanobacterium Synechococcus elongatus

Yang, Yiling; Lam, Vinson; Adomako, Marie; Simkovsky, Ryan; Jakob, Annik; Rockwell, Nathan C.; Cohen, Susan E.; Taton, Arnaud; Wang, Jingtong; Lagarias, J. Clark; Wilde, Annegret; Nobles, David R.; Brand, Jerry J.; Golden, Susan S.

doi:10.1073/pnas.1812871115

Title: Phototaxis in a wild isolate of the cyanobacterium Synechococcus elongatus

Journal Article · Fri Dec 14 00:00:00 EST 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1812871115· OSTI ID:1486917

Yang, Yiling; Lam, Vinson; Adomako, Marie;

; Jakob, Annik; Rockwell, Nathan C.; Cohen, Susan E.; Taton, Arnaud; Wang, Jingtong;

; Wilde, Annegret; Nobles, David R.; Brand, Jerry J.;

Many cyanobacteria, which use light as an energy source via photosynthesis, have evolved the ability to guide their movement toward or away from a light source. This process, termed “phototaxis,” enables organisms to localize in optimal light environments for improved growth and fitness. Mechanisms of phototaxis have been studied in the coccoid cyanobacterium Synechocystis sp. strain PCC 6803, but the rod-shaped Synechococcus elongatus PCC 7942, studied for circadian rhythms and metabolic engineering, has no phototactic motility. In this study we report a recent environmental isolate of S. elongatus, the strain UTEX 3055, whose genome is 98.5% identical to that of PCC 7942 but which is motile and phototactic. A six-gene operon encoding chemotaxis-like proteins was confirmed to be involved in phototaxis. Environmental light signals are perceived by a cyanobacteriochrome, PixJ_Se (Synpcc7942_0858), which carries five GAF domains that are responsive to blue/green light and resemble those of PixJ from Synechocystis. Furthermore, plate-based phototaxis assays indicate that UTEX 3055 uses PixJ_Se to sense blue and green light. Mutation of conserved functional cysteine residues in different GAF domains indicates that PixJ_Se controls both positive and negative phototaxis, in contrast to the multiple proteins that are employed for implementing bidirectional phototaxis in Synechocystis.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Institutes of Health (NIH); National Science Foundation (NSF); US‐Israel Binational Science Foundation; German Research Foundation (DFG)

Grant/Contract Number:: FG02-09ER16117; SC0002395; R35 GM118290; NSF‐BSF 2012823; 1201881

OSTI ID:: 1486917

Alternate ID(s):: OSTI ID: 1610706

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 115 Journal Issue: 52; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 38 works

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59 BASIC BIOLOGICAL SCIENCES
science & technology - other topics
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photoreceptor
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phototaxis
Synechococcus elongatus

Title: Phototaxis in a wild isolate of the cyanobacterium Synechococcus elongatus

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