High-throughput identification of novel heat tolerance genes via genome-wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii

Mattoon, Erin M.; McHargue, William E.; Bailey, Catherine E.; Zhang, Ningning; Chen, Chen; Eckhardt, James; Daum, Chris G.; Zane, Matt; Pennacchio, Christa; Schmutz, Jeremy; O'Malley, Ronan C.; Cheng, Jianlin; Zhang, Ru

doi:10.1111/pce.14507

Title: High-throughput identification of novel heat tolerance genes via genome-wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii

Journal Article · Wed Dec 07 00:00:00 EST 2022 · Plant, Cell and Environment

DOI:https://doi.org/10.1111/pce.14507· OSTI ID:2308836

^[1]; McHargue, William E. ^[2]; Bailey, Catherine E. ^[2];

^[2]; Chen, Chen ^[3]; Eckhardt, James ^[2]; Daum, Chris G. ^[4]; Zane, Matt ^[4]; Pennacchio, Christa ^[4];

^[5]; O'Malley, Ronan C. ^[6];

^[3];

^[2]

Donald Danforth Plant Science Center, St. Louis, MO (United States); Washington Univ., St. Louis, MO (United States)
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Univ. of Missouri, Columbia, MO (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Joint Genome Institute and Environmental Genomics and Systems Biology Division

Different high temperatures adversely affect crop and algal yields with various responses in photosynthetic cells. The list of genes required for thermotolerance remains elusive. Additionally, it is unclear how carbon source availability affects heat responses in plants and algae. Here, we utilized the insertional, indexed, genome-saturating mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii to perform genome-wide, quantitative, pooled screens under moderate (35°C) or acute (40°C) high temperatures with or without organic carbon sources. We identified heat-sensitive mutants based on quantitative growth rates and identified putative heat tolerance genes (HTGs). By triangulating HTGs with heat-induced transcripts or proteins in wildtype cultures and MapMan functional annotations, we presented a high/medium-confidence list of 933 Chlamydomonas genes with putative roles in heat tolerance. Triangulated HTGs include those with known thermotolerance roles and novel genes with little or no functional annotation. About 50% of these high-confidence HTGs in Chlamydomonas have orthologs in green lineage organisms, including crop species. Arabidopsis thaliana mutants deficient in the ortholog of a high-confidence Chlamydomonas HTG were also heat sensitive. This work expands our knowledge of heat responses in photosynthetic cells and provides engineering targets to improve thermotolerance in algae and crops.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: SC0020400; AC02-05CH11231

OSTI ID:: 2308836

Alternate ID(s):: OSTI ID: 2318527

Journal Information:: Plant, Cell and Environment, Vol. 46, Issue 3; ISSN 0140-7791

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA Zhang, Ru; Patena, Weronika; Armbruster, Ute The Plant Cell, Vol. 26, Issue 4 https://doi.org/10.1105/tpc.114.124099	journal	April 2014
Photosynthetic electron transport and proton flux under moderate heat stress Zhang, Ru; Sharkey, Thomas D. Photosynthesis Research, Vol. 100, Issue 1 https://doi.org/10.1007/s11120-009-9420-8	journal	April 2009
Temperature increase reduces global yields of major crops in four independent estimates Zhao, Chuang; Liu, Bing; Piao, Shilong Proceedings of the National Academy of Sciences, Vol. 114, Issue 35 https://doi.org/10.1073/pnas.1701762114	journal	August 2017
Comparative Proteomics Analysis of the Root Apoplasts of Rice Seedlings in Response to Hydrogen Peroxide Zhou, Lu; Bokhari, Saleem A.; Dong, Chun-Juan PLoS ONE, Vol. 6, Issue 2 https://doi.org/10.1371/journal.pone.0016723	journal	February 2011

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59 BASIC BIOLOGICAL SCIENCES
acute high temperature
Chlamydomonas reinhardtii
CLiP mutant library
DNA barcodes
heat responses
heat tolerance genes (HTGs)
moderate high temperature
photobioreactors (PBRs)
quantitative pooled screens

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