Genetic map of the fused locus on mouse Chromosome 17

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1475· OSTI ID:186089

Rossi, J M; Chen, Hsiuchen; Tilghman, S M ^[1]

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1. Princeton Univ., NJ (United States)

Fused (Fu) is a dominant mutation in mice resulting in the asymmetry and fusion of tail vertebrae in heterozygotes. Fu/Fu homozygotes are often viable and can exhibit a duplication of the terminal tail vertebrae resulting in bifurcated tails. There are two more severe alleles at Fu, Kinky (Fu{sup Ki}) and Knobbly (Fu{sup Kb)}, which die between 9 and 10 days of gestation as homozygotes, exhibiting a duplication of the embryonic axis, leading to incomplete or complete twinning. To define the precise map position of the Fu{sup Ki} mutation on mouse Chromosome 17, a 983-animal (Fu{sup Ki} if x Mus spretus) F{sub 1} x + tfl + tf interspecific backcross was generated and scored for Fu{sup Ki}, another tightly linked visible marker tufted (tf), and five linked molecular loci, D17MIT18, D17Leh54, D17Aus57, Hba-ps4, and Pim1. The order and genetic distances between the markers were determined to be centromere-D17MIT18-5.79 cM-D17Leh54-0.85 cM-D17Pri6-0.12 cM-D17Pri7-0.12 eM-Hba-ps4-1.20 cM-D17Pri8-0.48 cM-tf-2.05 cM-Pim1. The Fu{sup Ki} gene could not be genetically separated from three molecular markers, D17Pri6, D17Pri7, and Hba-ps4. Yeasts artificial chromosome clones that contain these tightly linked markers have been isolated to form a contig that contains Fu{sup Ki}. Recombination breakpoints generated through the interspecies backcross were mapped onto the contig and demonstrate that recombination in this region is not random.

• OSTI ID: 186089
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 23; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMES
CONTIGS
DNA-CLONING
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC MAPPING
GENOTYPE
HYBRIDIZATION
MICE
POLYMERASE CHAIN REACTION
YEASTS