Chromosomal mapping of the structural gene coding for the mouse cell adhesion molecule uvomorulin

Journal Article · Sun May 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.10.3489· OSTI ID:5481542

Eistetter, H R; Adolph, S; Ringwald, M; Simon-Chazottes, D; Schuh, R; Guenet, J L; Kemler, R ^[1]

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1. Max-Planck-Gesellschaft, Tuebingen (West Germany)

The gene coding for the mouse cell adhesion molecule uvomorulin has been mapped to chromosome 8. Uvomorulin cDNA clone F5H3 identified restriction fragment length polymorphisms in Southern blots of genomic DNA from mouse species Mus musculus domesticus and Mus spretus. By analyzing the segregation pattern of the gene in 75 offspring from an interspecific backcross a single genetic locus, Um, was defined on chromosome 8. Recombination frequency between Um and the co-segregating loci serum esterase 1 (Es-1) and tyrosine aminotransferase (Tat) places Um about 14 centimorgan (cM) distal to Es-1, and 5 cM proximal to Tat. In situ hybridization of uvomorulin ({sup 3}H)cDNA to mouse metaphase chromosomes located the Um locus close to the distal end of chromosome 8 (bands C3-E1). Since uvomorulin is evolutionarily highly conserved, its chromosomal assignment adds an important marker to the mouse genetic map.

• OSTI ID: 5481542
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:10; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
DNA
GENE RECOMBINATION
GENES
GENETIC MAPPING
HYBRIDIZATION
HYDROGEN COMPOUNDS
MAMMALS
MAPPING
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECOMBINANT DNA
RFLPS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES