Co-flow injection for serial crystallography at X-ray free-electron lasers

Doppler, Diandra; Rabbani, Mohammad T.; Letrun, Romain; Cruz Villarreal, Jorvani; Kim, Dai Hyun; Gandhi, Sahir; Egatz-Gomez, Ana; Sonker, Mukul; Chen, Joe; Koua, Faisal M.; Yang, Jayhow; Youssef, Mohamed; Mazalova, Victoria; Bajt, Saša; Shelby, Megan L.; Coleman, Matt A.; Wiedorn, Max O.; Knoska, Juraj; Schön, Silvan; Sato, Tokushi; Hunter, Mark S.; Hosseinizadeh, Ahmad; Kuptiz, Christopher; Nazari, Reza; Alvarez, Roberto C.; Karpos, Konstantinos; Zaare, Sahba; Dobson, Zachary; Discianno, Erin; Zhang, Shangji; Zook, James D.; Bielecki, Johan; de Wijn, Raphael; Round, Adam R.; Vagovic, Patrik; Kloos, Marco; Vakili, Mohammad; Ketawala, Gihan K.; Stander, Natasha E.; Olson, Tien L.; Morin, Katherine; Mondal, Jyotirmory; Nguyen, Jonathan; Meza-Aguilar, José Domingo; Kodis, Gerdenis; Vaiana, Sara; Martin-Garcia, Jose M.; Mariani, Valerio; Schwander, Peter; Schmidt, Marius; Messerschmidt, Marc; Ourmazd, Abbas; Zatsepin, Nadia; Weierstall, Uwe; Bruce, Barry D.; Mancuso, Adrian P.; Grant, Thomas; Barty, Anton; Chapman, Henry N.; Frank, Matthias; Fromme, Raimund; Spence, John C. H.; Botha, Sabine; Fromme, Petra; Kirian, Richard A.; Ros, Alexandra

doi:10.1107/s1600576721011079

Co-flow injection for serial crystallography at X-ray free-electron lasers

Journal Article · Tue Feb 01 04:00:00 EST 2022 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/s1600576721011079· OSTI ID:1856527

^[1]; ^[1]; ^[2]; ^[1]; Kim, Dai Hyun ^[1]; Gandhi, Sahir ^[1]; Egatz-Gomez, Ana ^[1]; Sonker, Mukul ^[1]; Chen, Joe ^[3]; ^[4]; Yang, Jayhow ^[3]; Youssef, Mohamed ^[4]; Mazalova, Victoria ^[4]; ^[5]; ^[6]; Coleman, Matt A. ^[6]; ^[7]; ^[4]; Schön, Silvan ^[4]; ^[2] more »

Arizona State Univ., Tempe, AZ (United States); Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Center for Applied Structural Discovery
European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
Arizona State Univ., Tempe, AZ (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
Hamburg Center for Ultrafast Imaging (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Hamburg Center for Ultrafast Imaging (Germany); Univ. Hamburg (Germany)
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Univ. of Wisconsin, Milwaukee, WI (United States)
Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Center for Applied Structural Discovery
European X-ray Free-Electron Laser (XFEL), Hamburg (Germany); Keele Univ., Staffordshire (United Kingdom)
European X-ray Free-Electron Laser (XFEL), Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
Univ. of Tennessee, Knoxville, TN (United States)
Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Center for Applied Structural Discovery; European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Center for Applied Structural Discovery; Consejo Superior de Investigaciones Cientificas (CSIC), Madrid (Spain). Inst. of Physical Chemistry
Arizona State Univ., Tempe, AZ (United States); Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Center for Applied Structural Discovery; La Trobe Univ., Melbourne, VIC (Australia). La Trobe Inst. for Molecular Science
European X-ray Free-Electron Laser (XFEL), Hamburg (Germany); La Trobe Univ., Melbourne, VIC (Australia). La Trobe Inst. for Molecular Science
State Univ. of New York (SUNY), Buffalo, NY (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Data and Computing in Natural Science (CDCS)

Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macromolecules at room temperature. Despite the impressive exposition of structural details with this novel crystallographic approach, the methods currently available to introduce crystals into the path of the X-ray beam sometimes exhibit serious drawbacks. Samples requiring liquid injection of crystal slurries consume large quantities of crystals (at times up to a gram of protein per data set), may not be compatible with vacuum configurations on beamlines or provide a high background due to additional sheathing liquids present during the injection. Proposed and characterized here is the use of an immiscible inert oil phase to supplement the flow of sample in a hybrid microfluidic 3D-printed co-flow device. Co-flow generation is reported with sample and oil phases flowing in parallel, resulting in stable injection conditions for two different resin materials experimentally. A numerical model is presented that adequately predicts these flow-rate conditions. The co-flow generating devices reduce crystal clogging effects, have the potential to conserve protein crystal samples up to 95% and will allow degradation-free light-induced time-resolved SFX.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Institutes of Health (NIH); German Research Foundation (DFG)

Grant/Contract Number:: AC02-76SF00515; SC0002164

OSTI ID:: 1856527

Alternate ID(s):: OSTI ID: 1885112

Journal Information:: Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 1 Vol. 55; ISSN 1600-5767

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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74 ATOMIC AND MOLECULAR PHYSICS
X-ray free-electron lasers
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Co-flow injection for serial crystallography at X-ray free-electron lasers

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