Nanoflow electrospinning serial femtosecond crystallography

Journal Article · Thu Nov 01 04:00:00 EDT 2012 · Acta Crystallographica. Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S0907444912038152· OSTI ID:22351266

Sierra, Raymond G.; Laksmono, Hartawan ^[1]; Kern, Jan ^[2]; Tran, Rosalie; Hattne, Johan ^[2]; Alonso-Mori, Roberto ^[1]; Lassalle-Kaiser, Benedikt ^[2]; Glöckner, Carina; Hellmich, Julia ^[3]; Schafer, Donald W. ^[1]; Echols, Nathaniel; Gildea, Richard J.; Grosse-Kunstleve, Ralf W. ^[2]; Sellberg, Jonas ^[1]; McQueen, Trevor A. ^[4]; Fry, Alan R.; Messerschmidt, Marc M.; Miahnahri, Alan; Seibert, M. Marvin; Hampton, Christina Y. ; Starodub, Dmitri; Loh, N. Duane; Sokaras, Dimosthenis; Weng, Tsu-Chien ^[1]; Zwart, Petrus H. ^[2]; Glatzel, Pieter ^[5]; Milathianaki, Despina; White, William E. ^[1]; Adams, Paul D. ^[2]; Williams, Garth J.; Boutet, Sébastien ^[1]; Zouni, Athina ^[3]; Messinger, Johannes ^[6]; Sauter, Nicholas K. ^[2]; Bergmann, Uwe ^[1]; Yano, Junko; Yachandra, Vittal K. ^[2]

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1. SLAC National Accelerator Laboratory, Menlo Park, CA 94025 (United States)
2. Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)
3. Technische Universität Berlin, Strasse des 17 Juni 135, 10623 Berlin (Germany)
4. Stanford University, Stanford, CA 94025 (United States)
5. European Synchrotron Radiation Facility, Grenoble (France)
6. Umeå Universitet, Umeå (Sweden)

A low flow rate liquid microjet method for delivery of hydrated protein crystals to X-ray lasers is presented. Linac Coherent Light Source data demonstrates serial femtosecond protein crystallography with micrograms, a reduction of sample consumption by orders of magnitude. An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min{sup −1} to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min{sup −1} and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

• OSTI ID: 22351266
• Journal Information: Acta Crystallographica. Section D: Biological Crystallography, Journal Name: Acta Crystallographica. Section D: Biological Crystallography Journal Issue: Pt 11 Vol. 68; ISSN ABCRE6; ISSN 0907-4449
• Country of Publication: Denmark
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLOGRAPHY
CRYSTALS
DELIVERY
DIFFRACTION
FLOW RATE
LIGHT SOURCES
LINEAR ACCELERATORS
LIQUIDS
PROTEINS
REDUCTION
RESOLUTION
SPIN
SUSPENSIONS
X-RAY LASERS