Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery

Beyerlein, K. R.; Heymann, M.; Kirian, R.; Adriano, L.; Knoška, J.; Wilde, F.; Chapman, H. N.

doi:10.1063/1.4936843

Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery

Journal Article · Tue Dec 15 04:00:00 EST 2015 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.4936843· OSTI ID:22482669

Beyerlein, K. R.; Heymann, M.; Kirian, R. ^[1]; Adriano, L.; Knoška, J. ^[2]; Wilde, F. ^[3]; Chapman, H. N. ^[1]

Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestraße 85, 22607 Hamburg (Germany)
Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg (Germany)
Helmholtz-Zentrum Geesthacht, Max-Planck-Straße 1, 21502 Geesthacht (Germany)

Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquid flow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.

OSTI ID:: 22482669

Journal Information:: Review of Scientific Instruments, Journal Name: Review of Scientific Instruments Journal Issue: 12 Vol. 86; ISSN 0034-6748; ISSN RSINAK

Country of Publication:: United States

Language:: English

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