LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis

Pyc, Michal; Gidda, Satinder K.; Seay, Damien; Esnay, Nicolas; Kretzschmar, Franziska K.; Cai, Yingqi; Doner, Nathan M.; Greer, Michael S.; Hull, J. Joe; Coulon, Denis; Bréhélin, Claire; Yurchenko, Olga; de Vries, Jan; Valerius, Oliver; Braus, Gerhard H.; Ischebeck, Till; Chapman, Kent D.; Dyer, John M.; Mullen, Robert T.

doi:10.1093/plcell/koab179

LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis

Journal Article · Wed Jun 09 04:00:00 EDT 2021 · The Plant Cell

DOI:https://doi.org/10.1093/plcell/koab179· OSTI ID:1852282

^[1]; ^[2]; ^[3]; ^[4]; ^[5]; ^[6]; ^[2]; ^[7]; ^[3]; ^[8]; Bréhélin, Claire ^[8]; ^[9]; ^[5]; ^[5]; ^[5]; ^[5]; ^[4]; ^[10]; ^[2]

Univ. of Guelph, ON (Canada); Willow Biosciences, Burnaby, BC (Canada); OSTI
Univ. of Guelph, ON (Canada)
US Dept. of Agriculture (USDA), Maricopa, AZ (United States). ARS-Arid-Land Agricultural Research Center
Univ. of North Texas, Denton, TX (United States)
Gottingen Univ. (Germany)
Univ. of North Texas, Denton, TX (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of North Texas, Denton, TX (United States); Willow Biosciences, Calgary, AB (Canada)
Université de Bordeaux, Villenave d’Ornon (France). Centre National de la Recherche Scientifique (CNRS)
US Dept. of Agriculture (USDA), Maricopa, AZ (United States). ARS-Arid-Land Agricultural Research Center; Purdue Univ., West Lafayette, IN (United States)
US Dept. of Agriculture (USDA), Maricopa, AZ (United States). ARS-Arid-Land Agricultural Research Center; US Dept. of Agriculture (USDA), Albany, CA (United States). Agricultural Research Service (ARS)

Cytoplasmic lipid droplets (LDs) are evolutionarily conserved organelles that store neutral lipids and play critical roles in plant growth, development, and stress responses. However, the molecular mechanisms underlying their biogenesis at the endoplasmic reticulum (ER) remain obscure. Here we show that a recently identified protein termed LD-associated protein [LDAP]-interacting protein (LDIP) works together with both endoplasmic reticulum-localized SEIPIN and the LD-coat protein LDAP to facilitate LD formation in Arabidopsis thaliana. Heterologous expression in insect cells demonstrated that LDAP is required for the targeting of LDIP to the LD surface, and both proteins are required for the production of normal numbers and sizes of LDs in plant cells. LDIP also interacts with SEIPIN via a conserved hydrophobic helix in SEIPIN and LDIP functions together with SEIPIN to modulate LD numbers and sizes in plants. Further, the co-expression of both proteins is required to restore normal LD production in SEIPIN-deficient yeast cells. These data, combined with the analogous function of LDIP to a mammalian protein called LD Assembly Factor 1, are discussed in the context of a new model for LD biogenesis in plant cells with evolutionary connections to LD biogenesis in other eukaryotes.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDA; Natural Sciences and Engineering Research Council of Canada (NSERC); German Research Foundation (DFG); European Research Council (ERC); French National Centre for Scientific Research

Grant/Contract Number:: SC0016536

OSTI ID:: 1852282

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 9 Vol. 33; ISSN 1040-4651

Publisher:: American Society of Plant Biologists (ASPB)Copyright Statement

Country of Publication:: United States

Language:: English

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Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging Yang, Huei-Jiun; Hsu, Chia-Ling; Yang, Jin-Yi PLoS ONE, Vol. 7, Issue 3 https://doi.org/10.1371/journal.pone.0032693	journal	March 2012
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Comparative Lipidomics and Proteomics of Lipid Droplets in the Mesocarp and Seed Tissues of Chinese Tallow (Triadica sebifera) Zhi, Yao; Taylor, Matthew C.; Campbell, Peter M. Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01339	journal	August 2017
New Insights Into the Role of Seed Oil Body Proteins in Metabolism and Plant Development Shao, Qun; Liu, Xiaofan; Su, Tong Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.01568	journal	December 2019
Promethin Is a Conserved Seipin Partner Protein Castro, Inês; Eisenberg-Bord, Michal; Persiani, Elisa Cells, Vol. 8, Issue 3 https://doi.org/10.3390/cells8030268	journal	March 2019
Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells Gidda, Satinder K.; Watt, Samantha C.; Collins-Silva, Jillian Plant Signaling & Behavior, Vol. 8, Issue 11 https://doi.org/10.4161/psb.27141	journal	November 2013
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Here, there and everywhere – the importance of neutral lipids in plant growth and development Zienkiewicz, Agnieszka; Saldat, Marta; Zienkiewicz, Krzysztof Postępy Biochemii https://doi.org/10.18388/pb.2021_409	journal	November 2021

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