Arabidopsis lipid droplet‐associated protein (LDAP) – interacting protein ( LDIP ) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds

Pyc, Michal; Cai, Yingqi; Gidda, Satinder K.; Yurchenko, Olga; Park, Sunjung; Kretzschmar, Franziska K.; Ischebeck, Till; Valerius, Oliver; Braus, Gerhard H.; Chapman, Kent D.; Dyer, John M.; Mullen, Robert T.

doi:10.1111/tpj.13754

Arabidopsis lipid droplet‐associated protein (LDAP) – interacting protein ( LDIP ) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds

Journal Article · Sun Nov 26 23:00:00 EST 2017 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.13754· OSTI ID:1410370

Pyc, Michal ^[1]; Cai, Yingqi ^[2]; Gidda, Satinder K. ^[1]; Yurchenko, Olga ^[3]; Park, Sunjung ^[3]; Kretzschmar, Franziska K. ^[4]; Ischebeck, Till ^[4]; Valerius, Oliver ^[5]; Braus, Gerhard H. ^[5]; Chapman, Kent D. ^[2]; Dyer, John M. ^[3]; Mullen, Robert T. ^[1]

Department of Molecular and Cellular Biology University of Guelph Guelph ON N1G 2W1 Canada
Department of Biological Sciences Center for Plant Lipid Research University of North Texas Denton TX 76203 USA
US Department of Agriculture Agricultural Research Service US Arid‐Land Agricultural Research Center Maricopa AZ 85138 USA
Department of Plant Biochemistry Albrecht‐von‐Haller‐Institute for Plant Sciences University of Goettingen Justus‐von‐Liebig‐Weg 11 37007 Goettingen Germany
Department of Molecular Microbiology and Genetics Institute for Microbiology and Genetics University of Goettingen Grisebachstrasse 8 37077 Goettingen Germany

Summary

Cytoplasmic lipid droplets ( LD s) are found in all types of plant cells; they are derived from the endoplasmic reticulum and function as a repository for neutral lipids, as well as serving in lipid remodelling and signalling. However, the mechanisms underlying the formation, steady‐state maintenance and turnover of plant LD s, particularly in non‐seed tissues, are relatively unknown. Previously, we showed that the LD ‐associated proteins ( LDAP s) are a family of plant‐specific, LD surface‐associated coat proteins that are required for proper biogenesis of LD s and neutral lipid homeostasis in vegetative tissues. Here, we screened a yeast two‐hybrid library using the Arabidopsis LDAP 3 isoform as ‘bait’ in an effort to identify other novel LD protein constituents. One of the candidate LDAP 3‐interacting proteins was Arabidopsis At5g16550, which is a plant‐specific protein of unknown function that we termed LDIP ( LDAP ‐interacting protein). Using a combination of biochemical and cellular approaches, we show that LDIP targets specifically to the LD surface, contains a discrete amphipathic α‐helical targeting sequence, and participates in both homotypic and heterotypic associations with itself and LDAP 3, respectively. Analysis of LDIP T‐ DNA knockdown and knockout mutants showed a decrease in LD abundance and an increase in variability of LD size in leaves, with concomitant increases in total neutral lipid content. Similar phenotypes were observed in plant seeds, which showed enlarged LD s and increases in total amounts of seed oil. Collectively, these data identify LDIP as a new player in LD biology that modulates both LD size and cellular neutral lipid homeostasis in both leaves and seeds.

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Sponsoring Organization:: USDOE

OSTI ID:: 1410370

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 6 Vol. 92; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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