Following O and OH in He/O 2 and He/H 2 O gas mixtures—from the gas phase through the liquid phase to modifications on a biological sample

Stapelmann, Katharina; Myers, Brayden; Quesada, Maria Herrera; Lenker, Eleanor; Ranieri, Pietro J.

doi:10.1088/1361-6463/ac18ec

Title: Following O and OH in He/O ₂ and He/H ₂ O gas mixtures—from the gas phase through the liquid phase to modifications on a biological sample

Journal Article · Mon Aug 09 00:00:00 EDT 2021 · Journal of Physics. D, Applied Physics

DOI:https://doi.org/10.1088/1361-6463/ac18ec· OSTI ID:1835357

; Myers, Brayden;

; Lenker, Eleanor;

Abstract Applied cold atmospheric plasma allows for the controlled delivery of reactive oxygen and nitrogen species tailored for specific applications. Through the manipulation of the plasma parameters, feed gases, and careful consideration of the environment surrounding the treatment target, selective chemistries that preferentially influence the target can be produced and delivered. To demonstrate this, the COST reference microscale atmospheric pressure plasma jet is used to study the generation and transport of O and $^{\cdot}$ OH from the gas phase through the liquid to the biological model target cysteine. Relative and absolute species densities of $^{\cdot}$ OH and O are measured in the gas phase through laser induced fluorescence (LIF) and two-photon absorption LIF respectively. The transport of these species is followed into the liquid phase by hydrogen peroxide quantification and visualized by a fluorescence assay. Modifications to the model biological sample cysteine exposed to $^{\cdot}$ OH and H ₂ O ₂ dominated chemistry (He/H ₂ O (0.25%)) and O dominated chemistry (He/O ₂ (0.6%)) is measured by FTIR spectroscopy. The origin of these species that modify cysteine is considered through the use of heavy water (H $_{2}^{18}$ O) and mass spectrometry. It is found that the reaction pathways differ significantly for He/O ₂ and He/H ₂ O. Hydrogen peroxide is formed mainly in the liquid phase in the presence of a substrate for He/O ₂ whereas for He/H ₂ O it forms in the gas phase. The liquid chemistry resulting from the He/O ₂ admixture mainly targets the sulfur moiety of cysteine for oxidation up to irreversible oxidation states, while He/H ₂ O treatment leads preferentially to reversible oxidation products. The more O or OH/H ₂ O ₂ dominated chemistry produced by the two gas admixtures studied offers the possibility to select species for target modification.

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Research Organization:: North Carolina State University, Raleigh, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Office of Workforce Development for Teachers & Scientists (WDTS); USDOE Office of Science (SC), Fusion Energy Sciences (FES); USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS)

Grant/Contract Number:: SC-0021329; SC0021329; SC0014664

OSTI ID:: 1835357

Alternate ID(s):: OSTI ID: 1830725

Journal Information:: Journal of Physics. D, Applied Physics, Journal Name: Journal of Physics. D, Applied Physics Vol. 54 Journal Issue: 43; ISSN 0022-3727

Publisher:: IOP PublishingCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: Following O and OH in He/O 2 and He/H 2 O gas mixtures—from the gas phase through the liquid phase to modifications on a biological sample

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Title: Following O and OH in He/O ₂ and He/H ₂ O gas mixtures—from the gas phase through the liquid phase to modifications on a biological sample