Identification and analysis of sugar transporters capable of co–transporting glucose and xylose simultaneously

Kuanyshev, Nurzhan; Deewan, Anshu; Jagtap, Sujit Sadashiv; Liu, Jingjing; Selvam, Balaji; Chen, Li‐Qing; Shukla, Diwakar; Rao, Christopher V.; Jin, Yong‐Su

doi:10.1002/biot.202100238

Identification and analysis of sugar transporters capable of co–transporting glucose and xylose simultaneously

Journal Article · Sat Aug 21 00:00:00 EDT 2021 · Biotechnology Journal

DOI:https://doi.org/10.1002/biot.202100238· OSTI ID:1834458

Kuanyshev, Nurzhan ^[1]; ^[2]; Jagtap, Sujit Sadashiv ^[2]; Liu, Jingjing ^[2]; Selvam, Balaji ^[2]; Chen, Li‐Qing ^[2]; Shukla, Diwakar ^[2]; Rao, Christopher V. ^[2]; ^[2]

Univ. of Illinois at Urbana-Champaign, IL (United States); University of Illinois
Univ. of Illinois at Urbana-Champaign, IL (United States)

Simultaneous co-fermentation of glucose and xylose is a key desired trait of engineered Saccharomyces cerevisiae for efficient and rapid production of biofuels and chemicals. However, glucose strongly inhibits xylose transport by endogenous hexose transporters of S. cerevisiae. We identified structurally distant sugar transporters (Lipomyces starkeyi LST1_205437 and Arabidopsis thaliana AtSWEET7) capable of co-transporting glucose and xylose from previously unexplored oleaginous yeasts and plants. Kinetic analysis showed that LST1_205437 had lenient glucose inhibition on xylose transport and AtSWEET7 transported glucose and xylose simultaneously with no inhibition. Modelling studies of LST1_205437 revealed that Ala335 residue at sugar binding site can accommodates both glucose and xylose. Docking studies with AtSWEET7 revealed that Trp59, Trp183, Asn145, and Asn179 residues stabilized the interactions with sugars, allowing both xylose and glucose to be co-transported. In addition, we altered sugar preference of LST1_205437 by single amino acid mutation at Asn365. Furthermore, our findings provide a new mechanistic insight on glucose and xylose transport mechanism of sugar transporters and the identified sugar transporters can be employed to develop engineered yeast strains for producing cellulosic biofuels and chemicals.

View Accepted Manuscript (DOE)

Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 1834458

Journal Information:: Biotechnology Journal, Journal Name: Biotechnology Journal Journal Issue: 11 Vol. 16; ISSN 1860-6768

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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