Systems analysis of Lipomyces starkeyi during growth on various plant-based sugars

Deewan, Anshu; Liu, Jing-Jing; Jagtap, Sujit Sadashiv; Yun, Eun Ju; Walukiewicz, Hanna; Jin, Yong-Su; Rao, Christopher V.

doi:10.1007/s00253-022-12084-w

Systems analysis of Lipomyces starkeyi during growth on various plant-based sugars

Journal Article · Sat Jul 30 00:00:00 EDT 2022 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-022-12084-w· OSTI ID:1902747

Deewan, Anshu ^[1]; Liu, Jing-Jing ^[2]; Jagtap, Sujit Sadashiv ^[2]; Yun, Eun Ju ^[3]; Walukiewicz, Hanna ^[2]; Jin, Yong-Su ^[2]; ^[2]

Univ. of Illinois at Urbana-Champaign, IL (United States); University of Illinois
Univ. of Illinois at Urbana-Champaign, IL (United States)
Univ. of Illinois at Urbana-Champaign, IL (United States); Korea Univ., Seoul (Korea, Republic of)

Oleaginous yeasts have received significant attention due to their substantial lipid storage capability. The accumulated lipids can be utilized directly or processed into various bioproducts and biofuels. Lipomyces starkeyi is an oleaginous yeast capable of using multiple plant-based sugars, such as glucose, xylose, and cellobiose. It is, however, a relatively unexplored yeast due to limited knowledge about its physiology. In this study, we have evaluated the growth of L. starkeyi on different sugars and performed transcriptomic and metabolomic analyses to understand the underlying mechanisms of sugar metabolism. Principal component analysis showed clear differences resulting from growth on different sugars. We have further reported various metabolic pathways activated during growth on these sugars. We also observed non-specific regulation in L. starkeyi and have updated the gene annotations for the NRRL Y-11557 strain. Furthermore, this analysis provides a foundation for understanding the metabolism of these plant-based sugars and potentially valuable information to guide the metabolic engineering of L. starkeyi to produce bioproducts and biofuels.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018260; SC0018420

OSTI ID:: 1902747

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 17 Vol. 106; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Anshu Deewan and Jing-Jing Liu contributed equally to this work
Lipomyces starkeyi
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Oleaginous yeast
RNA sequencing
Sugar uptake
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Systems analysis of Lipomyces starkeyi during growth on various plant-based sugars

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