Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source

Patent · Tue Apr 20 04:00:00 EDT 2021

OSTI ID:1823870

Pfleger, Brian Frederick; Agnew, Daniel E.

Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.

• Research Organization: Univ. of Wisconsin, Madison, WI (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: FC02-07ER64494
• Assignee: Wisconsin Alumni Research Foundation (Madison, WI)
• Patent Number(s): 10,982,240
• Application Number: 15/915,964
• OSTI ID: 1823870
• Country of Publication: United States
• Language: English