Accurate in vivo tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS)

Journal Article · Fri Feb 19 00:00:00 EST 2021 · Theranostics

DOI:https://doi.org/10.7150/thno.53101· OSTI ID:1817030

Strobbia, Pietro ^[1]; Cupil-Garcia, Vanessa ^[2]; Crawford, Bridget M. ^[3]; Fales, Andrew M. ^[4]; Pfefer, T. Joshua ^[4]; Liu, Yang ^[3]; Maiwald, Martin ^[5]; Sumpf, Bernd ^[5]; Vo-Dinh, Tuan ^[6]

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1. Duke Univ., Durham, NC (United States). Fitzpatrick Inst. for Photonics; Duke Univ., Durham, NC (United States). Dept. of Biomedical Engineering; OSTI
2. Duke Univ., Durham, NC (United States). Fitzpatrick Inst. for Photonics; Duke Univ., Durham, NC (United States). Dept. of Chemistry
3. Duke Univ., Durham, NC (United States). Fitzpatrick Inst. for Photonics; Duke Univ., Durham, NC (United States). Dept. of Biomedical Engineering
4. U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Devices and Radiological Health
5. Leibniz Inst. for Höchstfrequenztechnik, Berlin (Germany). Ferdinand-Braun-Institut
6. Duke Univ., Durham, NC (United States). Fitzpatrick Inst. for Photonics; Duke Univ., Durham, NC (United States). Dept. of Biomedical Engineering; Duke Univ., Durham, NC (United States). Dept. of Chemistry

For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings. Methods: Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model. Results: We demonstrate for the first time the use of SERDS for in vivo tumor detection in a murine model under ambient light conditions. We compare traditional Raman detection with SERDS, showing that our method can improve sensitivity and accuracy for this task. Conclusion: Our results show that this method can be used to improve the accuracy and robustness of in vivo Raman/SERS biomedical application, aiding the process of clinical translation of these technologies.

• Research Organization: Duke Univ., Durham, NC (United States)
• Sponsoring Organization: European Commission; German Federal Ministry of Education and Research (BMBF); National Institutes of Health (NIH); USDOE Office of Science (SC)
• Grant/Contract Number: SC0019393
• OSTI ID: 1817030
• Journal Information: Theranostics, Journal Name: Theranostics Journal Issue: 9 Vol. 11; ISSN 1838-7640
• Publisher: Ivyspring
• Country of Publication: United States
• Language: English

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