Direct SERDS sensing of molecular biomarkers in plants under field conditions

Strobbia, Pietro; Odion, Ren A.; Maiwald, Martin; Sumpf, Bernd; Vo-Dinh, Tuan

doi:10.1007/s00216-020-02544-5

Direct SERDS sensing of molecular biomarkers in plants under field conditions

Journal Article · Mon Mar 09 00:00:00 EDT 2020 · Analytical and Bioanalytical Chemistry

DOI:https://doi.org/10.1007/s00216-020-02544-5· OSTI ID:1605338

Strobbia, Pietro ^[1]; Odion, Ren A. ^[2]; Maiwald, Martin ^[3]; Sumpf, Bernd ^[3]; Vo-Dinh, Tuan ^[2]

Duke Univ., Durham, NC (United States); Duke University
Duke Univ., Durham, NC (United States)
Leibniz-Inst. für Höchstfrequenztechnik, Berlin (Germany)

Molecular biomarkers such as microRNAs (miRNAs) play important roles in regulating various developmental processes in plants. Understanding these pathways will help bioengineer designing organisms for efficient biomass accumulation. Current methods for RNA analysis require sample extraction and multi-step sample analysis, hindering work in field studies. Recent work in the incorporation of nanomaterials for plant bioengineering research is leading the way of an agri-tech revolution. As an example, surface-enhanced Raman scattering (SERS)–based sensors can be used to monitor RNA in vivo. However, the use of SERS in the field has been limited due to issues with observing Raman signal over complex background. To this end, shifted-excitation Raman difference spectroscopy (SERDS) offers an effective solution to extract the SERS signal from high background based on a physical approach. In this manuscript, we report the first application of SERDS on SERS sensors. We investigated this technique on SERS sensor developed for the detection of a microRNA biomarker, miR858. We tested the technique on in vitro samples and validated the technique by detecting the presence of exogenous miR858 in plants directly under ambient light in a growth chamber. The possibility of moving the detection of nucleic acid targets outside the constraints of laboratory setting enables numerous important bioengineering applications. Finally, such applications can revolutionize biofuel production and agri-tech through the use of nanotechnology-based monitoring of plant growth, plant health, and exposure to pollution and pathogens.

View Accepted Manuscript (DOE)

Research Organization:: Duke Univ., Durham, NC (United States)

Sponsoring Organization:: Federal Ministry of Education and Research (BMBF); USDOE Office of Science (SC)

Grant/Contract Number:: SC0019393

OSTI ID:: 1605338

Journal Information:: Analytical and Bioanalytical Chemistry, Journal Name: Analytical and Bioanalytical Chemistry Vol. 412; ISSN 1618-2642

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
77 NANOSCIENCE AND NANOTECHNOLOGY
field conditions
miRNA
plants
sensing
shifted-excitation difference Raman spectroscopy
surface-enhanced Raman scattering

Direct SERDS sensing of molecular biomarkers in plants under field conditions

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