Dual activity of anthocyanidin reductase supports the dominant plant proanthocyanidin extension unit pathway

Jun, Ji Hyung; Lu, Nan; Docampo-Palacios, Maite; Wang, Xiaoqiang; Dixon, Richard A.

doi:10.1126/sciadv.abg4682

Dual activity of anthocyanidin reductase supports the dominant plant proanthocyanidin extension unit pathway

Journal Article · Sat May 01 00:00:00 EDT 2021 · Science Advances

DOI:https://doi.org/10.1126/sciadv.abg4682· OSTI ID:1816719

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Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. BioDiscovery Inst.; OSTI
Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. BioDiscovery Inst.

Proanthocyanidins (PAs) are plant natural products important for agriculture and human health. They are polymers of flavan-3-ol subunits, commonly (-)-epicatechin and/or (+)-catechin, but the source of the in planta extension unit that comprises the bulk of the polymer remains unclear, as does how PA composition is determined in different plant species. Anthocyanidin reductase (ANR) can generate 2,3-cis-epicatechin as a PA starter unit from cyanidin, which itself arises from 2,3-trans-leucocyanidin, but ANR proteins from different species produce mixtures of flavan-3-ols with different stereochemistries in vitro. Genetic and biochemical analyses here show that ANR has dual activity and is involved not only in the production of (-)-epicatechin starter units but also in the formation of 2,3-cis-leucocyanidin to serve as (-)-epicatechin extension units. Differences in the product specificities of ANRs account for the presence/absence of PA polymerization and the compositions of PAs across plant species.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Georgia, Athens, GA (United States); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0015662

OSTI ID:: 1816719

Alternate ID(s):: OSTI ID: 1963708

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 20 Vol. 7; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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