Immobilization of Methylosinus trichosporium OB3b for methanol production

Taylor, Anne; Molzahn, Paige; Bushnell, Tanner; Cheney, Clint; LaJeunesse, Monique; Azizian, Mohamad; Semprini, Lewis

doi:10.1007/s10295-018-2010-z

Immobilization of Methylosinus trichosporium OB3b for methanol production

Journal Article · Thu Mar 01 00:00:00 EST 2018 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-018-2010-z· OSTI ID:1773507

Taylor, Anne ^[1]; Molzahn, Paige ^[2]; Bushnell, Tanner ^[2]; Cheney, Clint ^[2]; LaJeunesse, Monique ^[2]; Azizian, Mohamad ^[2]; Semprini, Lewis ^[2]

0000 0001 2112 1969 grid.4391.f Department of Crop and Soil Science Oregon State University 3017 Agricultural Life Science Building 97331 Corvallis OR USA
0000 0001 2112 1969 grid.4391.f Department of Chemical Biological and Environmental Engineering Oregon State University Corvallis OR USA

Abstract

Due to the natural gas boom in North America, there is renewed interest in the production of other chemical products from methane. We investigated the feasibility of immobilizing the obligate methanotrophic bacterium Methylosinus trichosporium OB3b in alginate beads, and selectively inactivating methanol dehydrogenase (MDH) with cyclopropane to produce methanol. In batch cultures and in semi-continuous flow columns, the exposure of alginate-immobilized cells to cyclopropane or cyclopropanol resulted in the loss of the majority of MDH activity (> 80%), allowing methanol to accumulate to significant concentrations while retaining all of M. trichosporium OB3b’s methane monooxygenase capacity. Thereafter, the efficiency of methanol production fell due to recovery of most of the MDH activity; however, subsequent inhibition periods resulted in renewed methanol production efficiency, and immobilized cells retained methane-oxidizing activity for at least 14 days.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AR0000439

OSTI ID:: 1773507

Alternate ID(s):: OSTI ID: 1537709

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 3 Vol. 45; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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