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Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b

Journal Article · · Journal of Bacteriology
DOI:https://doi.org/10.1128/JB.01144-10· OSTI ID:1007842
 [1];  [2];  [2];  [3];  [4];  [5];  [6];  [7];  [6];  [8];  [9];  [9];  [8];  [8];  [10];  [11];  [12];  [10];  [9];  [9] more »;  [12];  [9];  [9];  [8];  [13] « less
  1. University of Alberta, Edmondton, Canada
  2. University of Michigan
  3. University of Iowa
  4. University of Warwick, UK
  5. Defence Science and Detection Department (Dstl), Wiltshire, UK
  6. University of Strasbourg
  7. University of Washington, Seattle
  8. Los Alamos National Laboratory (LANL)
  9. U.S. Department of Energy, Joint Genome Institute
  10. ORNL
  11. Radboud University Nijmegen, The Netherlands
  12. Genoscope/Centre National de la Recherche Scientifique-Unite Mixte de Recherche
  13. University of Louisville, Louisville
+ Show Author Affiliations
Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.
Research Organization:
Oak Ridge National Laboratory (ORNL)
Sponsoring Organization:
ORNL work for others
DOE Contract Number:
AC05-00OR22725
OSTI ID:
1007842
Journal Information:
Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 24 Vol. 192; ISSN 0021-9193
Country of Publication:
United States
Language:
English

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Related Subjects

03 NATURAL GAS
BIODEGRADATION
COPPER
CYTOCHROMES
ENZYMES
HYDROCARBONS
METHANE
METHYLOCOCCUS-CAPSULATUS BATH
OXIDATION
PARTICULATES
STRAINS