Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5921935

Tsien, Hsienchyang; Brusseau, G A; Hanson, R S; Wackett, L P ^[1]

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1. Univ. of Minnesota, Navarre (USA)

The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable of oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 {mu}M or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene.

• OSTI ID: 5921935
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:12; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BATCH CULTURE
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CONTINUOUS CULTURE
COPPER COMPOUNDS
DECOMPOSITION
ENZYMES
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
METABOLISM
METHANE
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
TRANSITION ELEMENT COMPOUNDS
VINYL CHLORIDE