Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Cai, Guangqin; Wang, Geliang; Kim, Sang-Chul; Li, Jianwu; Zhou, Yongming; Wang, Xuemin

doi:10.1186/s13068-021-01899-w

Title: Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Journal Article · Sat Feb 27 00:00:00 EST 2021 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-021-01899-w· OSTI ID:1768103

Cai, Guangqin; Wang, Geliang; Kim, Sang-Chul; Li, Jianwu; Zhou, Yongming;

Abstract Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Natural Science Foundation of China (NSFC); National Key Research and Development Program of China; Office of China Postdoctoral Council

Grant/Contract Number:: AR0000202; 31801029; 2016YFD0100506; 2017YFE0104800; 20160034

OSTI ID:: 1768103

Alternate ID(s):: OSTI ID: 1848177

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 14 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

References (42)

Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds Nguyen, Huu Tam; Park, Hyunwoo; Koster, Karen L. Plant Biotechnology Journal, Vol. 13, Issue 1 https://doi.org/10.1111/pbi.12233	journal	July 2014
Camelina uses, genetics, genomics, production, and management Berti, Marisol; Gesch, Russ; Eynck, Christina Industrial Crops and Products, Vol. 94 https://doi.org/10.1016/j.indcrop.2016.09.034	journal	December 2016
Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants Cai, Guangqin; Fan, Chuchuan; Liu, Sheng New Phytologist, Vol. 226, Issue 4 https://doi.org/10.1111/nph.16473	journal	March 2020
High level accumulation of EPA and DHA in field‐grown transgenic Camelina – a multi‐territory evaluation of TAG accumulation and heterogeneity Han, Lihua; Usher, Sarah; Sandgrind, Sjur Plant Biotechnology Journal, Vol. 18, Issue 11 https://doi.org/10.1111/pbi.13385	journal	May 2020
Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds Malik, Meghna R.; Yang, Wenyu; Patterson, Nii Plant Biotechnology Journal, Vol. 13, Issue 5 https://doi.org/10.1111/pbi.12290	journal	November 2014
GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein Fan, Chuchuan; Xing, Yongzhong; Mao, Hailiang Theoretical and Applied Genetics, Vol. 112, Issue 6 https://doi.org/10.1007/s00122-006-0218-1	journal	February 2006
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure Kagale, Sateesh; Koh, Chushin; Nixon, John Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4706	journal	April 2014
Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis Li, Maoyin; Bahn, Sung Chul; Guo, Liang The Plant Cell, Vol. 23, Issue 3 https://doi.org/10.1105/tpc.110.081240	journal	March 2011
Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop Ruiz-Lopez, Noemi; Haslam, Richard P.; Napier, Johnathan A. The Plant Journal, Vol. 77, Issue 2 https://doi.org/10.1111/tpj.12378	journal	December 2013
Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil Yu, Xiao-Hong; Cahoon, Rebecca E.; Horn, Patrick J. Plant Biotechnology Journal, Vol. 16, Issue 4 https://doi.org/10.1111/pbi.12839	journal	January 2018
Deletion in a gene associated with grain size increased yields during rice domestication Shomura, Ayahiko; Izawa, Takeshi; Ebana, Kaworu Nature Genetics, Vol. 40, Issue 8 https://doi.org/10.1038/ng.169	journal	July 2008
Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa Ozseyhan, Mehmet E.; Kang, Jinling; Mu, Xiaopeng Plant Physiology and Biochemistry, Vol. 123 https://doi.org/10.1016/j.plaphy.2017.11.021	journal	February 2018
AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum Kim, S.; Yamaoka, Y.; Ono, H. Proceedings of the National Academy of Sciences, Vol. 110, Issue 2 https://doi.org/10.1073/pnas.1214159110	journal	December 2012
Synthesis of oleyl oleate wax esters in Arabidopsis thaliana and Camelina sativa seed oil Iven, Tim; Hornung, Ellen; Heilmann, Mareike Plant Biotechnology Journal, Vol. 14, Issue 1 https://doi.org/10.1111/pbi.12379	journal	April 2015
Camelina seed transcriptome: a tool for meal and oil improvement and translational research Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram Plant Biotechnology Journal, Vol. 11, Issue 6 https://doi.org/10.1111/pbi.12068	journal	April 2013
Transcriptional Regulation of Lipid Catabolism during Seedling Establishment Cai, Guangqin; Kim, Sang-Chul; Li, Jianwu Molecular Plant, Vol. 13, Issue 7 https://doi.org/10.1016/j.molp.2020.04.007	journal	July 2020
Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight Weng, Jianfeng; Gu, Suhai; Wan, Xiangyuan Cell Research, Vol. 18, Issue 12 https://doi.org/10.1038/cr.2008.307	journal	November 2008
Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing Jiang, Wen Zhi; Henry, Isabelle M.; Lynagh, Peter G. Plant Biotechnology Journal, Vol. 15, Issue 5 https://doi.org/10.1111/pbi.12663	journal	January 2017
FAX1, a Novel Membrane Protein Mediating Plastid Fatty Acid Export Li, Nannan; Gügel, Irene Luise; Giavalisco, Patrick PLOS Biology, Vol. 13, Issue 2 https://doi.org/10.1371/journal.pbio.1002053	journal	February 2015
Field trial evaluation of the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Camelina sativa: Making fish oil substitutes in plants Usher, Sarah; Haslam, Richard P.; Ruiz-Lopez, Noemi Metabolic Engineering Communications, Vol. 2 https://doi.org/10.1016/j.meteno.2015.04.002	journal	December 2015
Genetic dissection of plant architecture and yield-related traits in Brassica napus Cai, Guangqin; Yang, Qingyong; Chen, Hao Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep21625	journal	February 2016
Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA Petrie, James R.; Shrestha, Pushkar; Belide, Srinivas PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0085061	journal	January 2014
Metabolic Engineering of Fatty Acid Biosynthesis in Plants Thelen, Jay J.; Ohlrogge, John B. Metabolic Engineering, Vol. 4, Issue 1 https://doi.org/10.1006/mben.2001.0204	journal	January 2002
Oil-seed crop: Camelina sativa Zubr, Josef Industrial Crops and Products, Vol. 6, Issue 2 https://doi.org/10.1016/S0926-6690(96)00203-8	journal	May 1997
Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds Abdullah, Hesham M.; Akbari, Parisa; Paulose, Bibin Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0555-5	journal	July 2016
Natural variation in GS5 plays an important role in regulating grain size and yield in rice Li, Yibo; Fan, Chuchuan; Xing, Yongzhong Nature Genetics, Vol. 43, Issue 12 https://doi.org/10.1038/ng.977	journal	October 2011
Plant acyl-CoA-binding proteins: An emerging family involved in plant development and stress responses Du, Zhi-Yan; Arias, Tatiana; Meng, Wei Progress in Lipid Research, Vol. 63 https://doi.org/10.1016/j.plipres.2016.06.002	journal	July 2016
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation Lu, Chaofu; Kang, Jinling Plant Cell Reports, Vol. 27, Issue 2 https://doi.org/10.1007/s00299-007-0454-0	journal	September 2007
Genetic and Molecular Bases of Rice Yield Xing, Yongzhong; Zhang, Qifa Annual Review of Plant Biology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev-arplant-042809-112209	journal	June 2010
Seed-specific overexpression of AtFAX1 increases seed oil content in Arabidopsis Tian, Yinshuai; Lv, Xueyan; Xie, Guilan Biochemical and Biophysical Research Communications, Vol. 500, Issue 2 https://doi.org/10.1016/j.bbrc.2018.04.081	journal	June 2018
Some compositional properties of camelina (camelina sativa L. Crantz) seeds and oils Budin, John T.; Breene, William M.; Putnam, Daniel H. Journal of the American Oil Chemists’ Society, Vol. 72, Issue 3 https://doi.org/10.1007/BF02541088	journal	March 1995
Biodiesel from alternative oilseed feedstocks: camelina and field pennycress Moser, Bryan R. Biofuels, Vol. 3, Issue 2 https://doi.org/10.4155/bfs.12.6	journal	March 2012
Diseases of Camelina sativa (false flax) Séguin-Swartz, G.; Eynck, C.; Gugel, R. K. Canadian Journal of Plant Pathology, Vol. 31, Issue 4 https://doi.org/10.1080/07060660909507612	journal	December 2009
A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase Song, Xian-Jun; Huang, Wei; Shi, Min Nature Genetics, Vol. 39, Issue 5 https://doi.org/10.1038/ng2014	journal	April 2007
Control of grain size, shape and quality by OsSPL16 in rice Wang, Shaokui; Wu, Kun; Yuan, Qingbo Nature Genetics, Vol. 44, Issue 8 https://doi.org/10.1038/ng.2327	journal	June 2012
Enhancing micro RNA 167A expression in seed decreases the α‐linolenic acid content and increases seed size in Camelina sativa Na, GunNam; Mu, Xiaopeng; Grabowski, Paul The Plant Journal, Vol. 98, Issue 2 https://doi.org/10.1111/tpj.14223	journal	February 2019
Acyl-Lipid Metabolism Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred The Arabidopsis Book, Vol. 11 https://doi.org/10.1199/tab.0161	journal	January 2013
An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds Ruiz-Lopez, Noemi; Haslam, Richard P.; Usher, Sarah Plant Biotechnology Journal, Vol. 13, Issue 9 https://doi.org/10.1111/pbi.12328	journal	January 2015
Profiling Membrane Lipids in Plant Stress Responses: ROLE OF PHOSPHOLIPASE Dα IN FREEZING-INDUCED LIPID CHANGES IN Welti, Ruth; Li, Weiqi; Li, Maoyin Journal of Biological Chemistry, Vol. 277, Issue 35 https://doi.org/10.1074/jbc.M205375200	journal	June 2002
Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value Liu, Jinjie; Rice, Adam; McGlew, Kathleen Plant Biotechnology Journal, Vol. 13, Issue 6 https://doi.org/10.1111/pbi.12325	journal	March 2015
The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3 Qi, Peng; Lin, You-Shun; Song, Xian-Jun Cell Research, Vol. 22, Issue 12 https://doi.org/10.1038/cr.2012.151	journal	November 2012
Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina Li, Maoyin; Wei, Fang; Tawfall, Amanda Plant Biotechnology Journal, Vol. 13, Issue 6 https://doi.org/10.1111/pbi.12304	journal	December 2014

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59 BASIC BIOLOGICAL SCIENCES
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