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Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina

Journal Article · · Plant Biotechnology Journal
DOI:https://doi.org/10.1111/pbi.12304· OSTI ID:1459197
 [1];  [2];  [2];  [2];  [2];  [2]
  1. Univ. of Missouri, St. Louis, MO (United States); Donald Danforth Plant Science Center, St. Louis, MO (United States); Donald Danforth Plant Science Center
  2. Univ. of Missouri, St. Louis, MO (United States); Donald Danforth Plant Science Center, St. Louis, MO (United States)
+ Show Author Affiliations
Camelina sativa is a Brassicaceae oilseed species being explored as a biofuel and industrial oil crop. A growing number of studies have indicated that the turnover of phosphatidylcholine plays an important role in the synthesis and modification of triacylglycerols. This study manipulated the expression of a patatin-related phospholipase AIIIδ (pPLAIIIδ) in camelina to determine its effect on seed oil content and plant growth. Constitutive overexpression of pPLAIIIδ under the control of the constitutive cauliflower mosaic 35S promoter resulted in a significant increase in seed oil content and a decrease in cellulose content. In addition, the content of major membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, in 35S::pPLAIIIδ plants was increased. However, these changes in 35S::pPLAIIIδ camelina were associated with shorter cell length, leaves, stems, and seed pods and a decrease in overall seed production. When pPLAIIIδ was expressed under the control of the seed specific, β-conglycinin promoter, the seed oil content was increased without compromising plant growth. In conclusion, the results suggest that pPLAIIIδ alters the carbon partitioning by decreasing cellulose content and increasing oil content in camelina.
Research Organization:
Donald Danforth Plant Science Center, St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Advanced Biofuel Systems (CABS)
Sponsoring Organization:
USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Grant/Contract Number:
SC0001295
OSTI ID:
1459197
Alternate ID(s):
OSTI ID: 1401833
OSTI ID: 1369677
Journal Information:
Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 6 Vol. 13; ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied BiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (6)

Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression journal November 2017
PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environments journal January 2019
Multi-Locus Genome-Wide Association Studies for 14 Main Agronomic Traits in Barley journal November 2018
Camelina sativa, an oilseed at the nexus between model system and commercial crop journal June 2018
Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds journal September 2019
Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase journal December 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
camelina
cellulose
patatin-related phospholipase IIIδ
plant growth
seed oil