Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants

Cai, Guangqin; Fan, Chuchuan; Liu, Sheng; Yang, Qingyong; Liu, Dongxu; Wu, Jian; Li, Jianwu; Zhou, Yongming; Guo, Liang; Wang, Xuemin

doi:10.1111/nph.16473

Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants

Journal Article · Mon Mar 16 00:00:00 EDT 2020 · New Phytologist

DOI:https://doi.org/10.1111/nph.16473· OSTI ID:1604837

^[1]; Fan, Chuchuan ^[2]; Liu, Sheng ^[2]; Yang, Qingyong ^[3]; Liu, Dongxu ^[3]; Wu, Jian ^[4]; Li, Jianwu ^[5]; Zhou, Yongming ^[2]; ^[2]; ^[6]

National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China, Department of Biology University of Missouri St Louis MO 63121 USA, Donald Danforth Plant Science Center St Louis MO 63132 USA
National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan Hubei 430070 China
Hubei Key Laboratory of Agricultural Bioinformatics College of Informatics Huazhong Agricultural University Wuhan Hubei 430070 China
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University Yangzhou Jiangsu 225009 China
Department of Biology University of Missouri St Louis MO 63121 USA, Donald Danforth Plant Science Center St Louis MO 63132 USA, Henan Agricultural University Zhengzhou Henan 450002 China
Department of Biology University of Missouri St Louis MO 63121 USA, Donald Danforth Plant Science Center St Louis MO 63132 USA

Summary

Plant oils are valuable commodities for food, feed, renewable industrial feedstocks and biofuels. To increase vegetable oil production, here we show that the nonspecific phospholipase C6 (NPC6) promotes seed oil production in the Brassicaceae seed oil species Arabidopsis, Camelina and oilseed rape.

Overexpression of NPC6 increased seed oil content, seed weight and oil yield both in Arabidopsis and Camelina, whereas knockout of NPC6 decreased seed oil content and seed size. NPC6 is associated with the chloroplasts and microsomal membranes, and hydrolyzes phosphatidylcholine and galactolipids to produce diacylglycerol. Knockout and overexpression of NPC6 decreased and increased, respectively, the flux of fatty acids from phospholipids and galactolipids into triacylglycerol production.

Candidate‐gene association study in oilseed rape indicates that only BnNPC6.C01 of the four homeologues NPC6s is associated with seed oil content and yield. Haplotypic analysis indicates that the BnNPC6.C01 favorable haplotype can increase both seed oil content and seed yield.

These results indicate that NPC6 promotes membrane glycerolipid turnover to accumulate TAG production in oil seeds and that NPC6 has a great application potential for oil yield improvement.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001295

OSTI ID:: 1604837

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 4 Vol. 226; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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