A transgene design for enhancing oil content in Arabidopsis and Camelina seeds

Zhu, Yerong; Xie, Linan; Chen, Grace Q.; Lee, Mi Yeon; Loque, Dominique; Scheller, Henrik Vibe

doi:10.1186/s13068-018-1049-4

Title: A transgene design for enhancing oil content in Arabidopsis and Camelina seeds

Journal Article · Wed Feb 21 00:00:00 EST 2018 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-018-1049-4· OSTI ID:1433120

Zhu, Yerong ^[1]; Xie, Linan ^[2]; Chen, Grace Q. ^[3]; Lee, Mi Yeon ^[4]; Loque, Dominique ^[5];

^[6]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Environmental Genomics and Systems Biology Division; Nankai Univ., Tianjin (China). College of Life Science
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Environmental Genomics and Systems Biology Division; Northeast Forestry Univ., Harbin (United States). College of Life Science
US Dept. of Agriculture (USDA)., Albany, CA (United States). Western Regional Research Center, Agricultural Research Service
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Environmental Genomics and Systems Biology Division
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Environmental Genomics and Systems Biology Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Environmental Genomics and Systems Biology Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology

Background: Increasing the oil yield is a major objective for oilseed crop improvement. Oil biosynthesis and accumulation are influenced by multiple genes involved in embryo and seed development. The leafy cotyledon1 (LEC1) is a master regulator of embryo development that also enhances the expression of genes involved in fatty acid biosynthesis. We speculated that seed oil could be increased by targeted overexpression of a master regulating transcription factor for oil biosynthesis, using a downstream promoter for a gene in the oil biosynthesis pathway. To verify the effect of such a combination on seed oil content, we made constructs with maize (Zea mays) ZmLEC1 driven by serine carboxypeptidase-like (SCPL17) and acyl carrier protein (ACP5) promoters, respectively, for expression in transgenic Arabidopsis thaliana and Camelina sativa. Results: Agrobacterium-mediated transformation successfully generated Arabidopsis and Camelina lines that overexpressed ZmLEC1 under the control of a seed-specific promoter. This overexpression does not appear to be detrimental to seed vigor under laboratory conditions and did not cause observable abnormal growth phenotypes throughout the life cycle of the plants. Overexpression of ZmLEC1 increased the oil content in mature seeds by more than 20% in Arabidopsis and 26% in Camelina. In conclusion: The findings suggested that the maize master regulator, ZmLEC1, driven by a downstream seed-specific promoter, can be used to increase oil production in Arabidopsis and Camelina and might be a promising target for increasing oil yield in oilseed crops.0

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); China Scholarship Council

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1433120

Journal Information:: Biotechnology for Biofuels, Vol. 11, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 20 works

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References (24)

Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis Plant Biotechnology Journal, Vol. 9, Issue 8, p. 874-883 https://doi.org/10.1111/j.1467-7652.2011.00599.x	journal	February 2011
LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development Stone, S. L.; Kwong, L. W.; Yee, K. M. Proceedings of the National Academy of Sciences, Vol. 98, Issue 20 https://doi.org/10.1073/pnas.201413498	journal	September 2001
WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis: Oil synthesis regulatory network in seeds Baud, Sébastien; Mendoza, Monica Santos; To, Alexandra The Plant Journal, Vol. 50, Issue 5 https://doi.org/10.1111/j.1365-313X.2007.03092.x	journal	April 2007
Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells Lotan, Tamar; Ohto, Masa-aki; Yee, Kelly Matsudaira Cell, Vol. 93, Issue 7 https://doi.org/10.1016/S0092-8674(00)81463-4	journal	June 1998
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter Vigeolas, Helene; Waldeck, Peter; Zank, Thorsten Plant Biotechnology Journal, Vol. 5, Issue 3 https://doi.org/10.1111/j.1467-7652.2007.00252.x	journal	May 2007
Engineering secondary cell wall deposition in plants Yang, Fan; Mitra, Prajakta; Zhang, Ling Plant Biotechnology Journal, Vol. 11, Issue 3 https://doi.org/10.1111/pbi.12016	journal	November 2012
An Expression and Bioinformatics Analysis of the Arabidopsis Serine Carboxypeptidase-Like Gene Family Fraser, Christopher M.; Rider, Lance W.; Chapple, Clint Plant Physiology, Vol. 138, Issue 2 https://doi.org/10.1104/pp.104.057950	journal	May 2005
ATTED-II provides coexpressed gene networks for Arabidopsis Obayashi, T.; Hayashi, S.; Saeki, M. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn807	journal	January 2009
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas Kim, Mi; Yang, Seong; Mao, Hui-Zhu Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-36	journal	January 2014
Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape ( Brassica napus L.) Kelly, Amélie A.; Shaw, Eve; Powers, Stephen J. Plant Biotechnology Journal, Vol. 11, Issue 3 https://doi.org/10.1111/pbi.12021	journal	November 2012
Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa An, Dahee; Suh, Mi Chung Plant Biotechnology Reports, Vol. 9, Issue 3 https://doi.org/10.1007/s11816-015-0351-x	journal	May 2015
Gateway-compatible vectors for plant functional genomics and proteomics Earley, Keith W.; Haag, Jeremy R.; Pontes, Olga The Plant Journal, Vol. 45, Issue 4 https://doi.org/10.1111/j.1365-313X.2005.02617.x	journal	February 2006
WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis Cernac, Alex; Benning, Christoph The Plant Journal, Vol. 40, Issue 4, p. 575-585 https://doi.org/10.1111/j.1365-313X.2004.02235.x	journal	September 2004
Enhanced Seed Oil Production in Canola by Conditional Expression of Brassica napus LEAFY COTYLEDON1 and LEC1-LIKE in Developing Seeds Tan, Helin; Yang, Xiaohui; Zhang, Fengxia Plant Physiology, Vol. 156, Issue 3 https://doi.org/10.1104/pp.111.175000	journal	May 2011
An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets Winter, Debbie; Vinegar, Ben; Nahal, Hardeep PLoS ONE, Vol. 2, Issue 8 https://doi.org/10.1371/journal.pone.0000718	journal	August 2007
Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis van Erp, Harrie; Kelly, Amélie A.; Menard, Guillaume Plant Physiology, Vol. 165, Issue 1 https://doi.org/10.1104/pp.114.236430	journal	April 2014
Expression of ZmLEC1 and ZmWRI1 Increases Seed Oil Production in Maize Shen, Bo; Allen, William B.; Zheng, Peizhong Plant Physiology, Vol. 153, Issue 3 https://doi.org/10.1104/pp.110.157537	journal	May 2010
Transcription factors as tools for metabolic engineering in plants Broun, Pierre Current Opinion in Plant Biology, Vol. 7, Issue 2 https://doi.org/10.1016/j.pbi.2004.01.013	journal	April 2004
Transcription factors for predictive plant metabolic engineering: are we there yet? Grotewold, Erich Current Opinion in Biotechnology, Vol. 19, Issue 2 https://doi.org/10.1016/j.copbio.2008.02.002	journal	April 2008
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight Jako, Colette; Kumar, Arvind; Wei, Yangdou Plant Physiology, Vol. 126, Issue 2 https://doi.org/10.1104/pp.126.2.861	journal	June 2001
Developmental control of Arabidopsis seed oil biosynthesis Wang, Hongyun; Guo, Jinhua; Lambert, Kris N. Planta, Vol. 226, Issue 3 https://doi.org/10.1007/s00425-007-0524-0	journal	May 2007
A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis Gondolf, Vibe M.; Stoppel, Rhea; Ebert, Berit BMC Plant Biology, Vol. 14, Issue 1 https://doi.org/10.1186/s12870-014-0344-x	journal	December 2014
Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls Vega-Sánchez, Miguel E.; Loqué, Dominique; Lao, Jeemeng Plant Biotechnology Journal, Vol. 13, Issue 7 https://doi.org/10.1111/pbi.12326	journal	January 2015

Cited By (4)

Mapping quantitative trait loci for seed traits in Camelina sativa King, Kevin; Li, Huang; Kang, Jinling Theoretical and Applied Genetics, Vol. 132, Issue 9 https://doi.org/10.1007/s00122-019-03371-8	journal	June 2019
Enhancing micro RNA 167A expression in seed decreases the α‐linolenic acid content and increases seed size in Camelina sativa Na, GunNam; Mu, Xiaopeng; Grabowski, Paul The Plant Journal, Vol. 98, Issue 2 https://doi.org/10.1111/tpj.14223	journal	February 2019
Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray) Stonebloom, Solomon H.; Scheller, Henrik Vibe BMC Plant Biology, Vol. 19, Issue 1 https://doi.org/10.1186/s12870-019-1669-2	journal	February 2019
WRINKLED1, a “Master Regulator” in Transcriptional Control of Plant Oil Biosynthesis Kong, Que; Yuan, Ling; Ma, Wei Plants, Vol. 8, Issue 7 https://doi.org/10.3390/plants8070238	journal	July 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ZmLEC1
Master regulator
Transcription factor
Oil content
SCPL17
ACP5

MOESM1 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914042 The current record is supplemented by this figure	figure	February 2018
MOESM2 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914066 The current record is supplemented by this dataset	dataset	February 2018
MOESM2 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914066.v1 The current record is supplemented by this dataset	dataset	February 2018
MOESM3 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914084 The current record is supplemented by this dataset	dataset	February 2018
MOESM3 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914084.v1 The current record is supplemented by this dataset	dataset	February 2018
MOESM4 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914096 The current record is supplemented by this dataset	dataset	February 2018
MOESM4 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914096.v1 The current record is supplemented by this dataset	dataset	February 2018
A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.c.4012546 The current record is supplemented by this dataset	dataset	February 2018
A transgene design for enhancing oil content in Arabidopsis and Camelina seeds [Supplementary Data] Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.c.4012546.v1 The current record is supplemented by this dataset	dataset	February 2018
MOESM1 of A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace https://doi.org/10.6084/m9.figshare.5914042.v1 The current record is supplemented by this dataset	dataset	February 2018