Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

Wang, Liang; Ou, Mark S.; Nieves, Ismael; Erickson, John E.; Vermerris, Wilfred; Ingram, L. O.; Shanmugam, K. T.

doi:10.1016/j.biortech.2015.09.062

Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

Journal Article · Sat Sep 26 00:00:00 EDT 2015 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2015.09.062· OSTI ID:1766805

Wang, Liang ^[1]; Ou, Mark S. ^[2]; Nieves, Ismael ^[2]; Erickson, John E. ^[2]; Vermerris, Wilfred ^[2]; Ingram, L. O. ^[2]; Shanmugam, K. T. ^[2]

Univ. of Florida, Gainesville, FL (United States); University of Florida
Univ. of Florida, Gainesville, FL (United States)

In this study, a moderate thermophile Clostridium thermobutyricum is shown to ferment the sugars in sweet sorghum juice treated with invertase and supplemented with tryptone (10 g L^–1) and yeast extract (10 g L^–1) at 50 °C to 44 g L^–1 butyrate at a calculated highest volumetric productivity of 1.45 g L^–1 h^–1 (molar butyrate yield of 0.85 based on sugars fermented). This volumetric productivity is among the highest reported for batch fermentations. Sugars from acid and enzyme-treated sweet sorghum bagasse were also fermented to butyrate by this organism with a molar yield of 0.81 (based on the amount of cellulose and hemicellulose). By combining the results from juice and bagasse, the calculated yield of butyric acid is approximately 90 kg per tonne of fresh sweet sorghum stalk. Here, this study demonstrates that C. thermobutyricum can be an effective microbial biocatalyst for production of bio-based butyrate from renewable feedstocks at 50 °C.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1766805

Alternate ID(s):: OSTI ID: 1771308
OSTI ID: 1250354

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: 198 Vol. 198; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

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