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Title: Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains

Abstract

Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3–7 Mg ha -1) and generates significant amounts of bagasse (15–20 Mg ha -1) as a lignocellulosic feedstock. These sugars can be fermented not only to biofuels but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the biodegradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice at 50 °C to L-(+)-lactic acid (~150 g L -1; productivity, 7.2 g L -1 h -1). B. coagulans strain QZ19-2 was used to ferment sorghum juice to d-(-)-lactic acid (~125 g L -1; productivity, 5 g L -1 h -1). Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5 % phosphoric acid at 190 °C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80 % of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain usedmore » as the microbial biocatalyst. Liquefaction of pretreated bagasse with cellulases before SScF (L + SScF) increased the productivity of lactic acid. Here, these results show that B. coagulans is an effective biocatalyst for fermentation of all the sugars present in sweet sorghum juice and bagasse to optically pure lactic acid at high titer and productivity as feedstock for bio-based plastics.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Univ. of Florida, Gainesville, FL (United States)
  2. Univ. of Florida, Gainesville, FL (United States). UF Genetics Inst.
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDA
OSTI Identifier:
1661477
Grant/Contract Number:  
PI0000031; 2012-67009-19596; 2011- 10006-30358
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
Bacillus coagulans; Fermentation; Lactic acid; Sorghumbicolor

Citation Formats

Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9670-6.
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., & Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States. doi:10.1007/s12155-015-9670-6.
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sun . "Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains". United States. doi:10.1007/s12155-015-9670-6. https://www.osti.gov/servlets/purl/1661477.
@article{osti_1661477,
title = {Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains},
author = {Ou, Mark S. and Awasthi, Deepika and Nieves, Ismael and Wang, Liang and Erickson, John and Vermerris, Wilfred and Ingram, L. O. and Shanmugam, K. T.},
abstractNote = {Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3–7 Mg ha-1) and generates significant amounts of bagasse (15–20 Mg ha-1) as a lignocellulosic feedstock. These sugars can be fermented not only to biofuels but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the biodegradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice at 50 °C to L-(+)-lactic acid (~150 g L-1; productivity, 7.2 g L-1 h-1). B. coagulans strain QZ19-2 was used to ferment sorghum juice to d-(-)-lactic acid (~125 g L-1; productivity, 5 g L-1 h-1). Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5 % phosphoric acid at 190 °C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80 % of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain used as the microbial biocatalyst. Liquefaction of pretreated bagasse with cellulases before SScF (L + SScF) increased the productivity of lactic acid. Here, these results show that B. coagulans is an effective biocatalyst for fermentation of all the sugars present in sweet sorghum juice and bagasse to optically pure lactic acid at high titer and productivity as feedstock for bio-based plastics.},
doi = {10.1007/s12155-015-9670-6},
journal = {BioEnergy Research},
issn = {1939-1234},
number = 1,
volume = 9,
place = {United States},
year = {2015},
month = {8}
}

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