Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

Wang, Liang; Ou, Mark S.; Nieves, Ismael; Erickson, John E.; Vermerris, Wilfred; Ingram, L. O.; Shanmugam, K. T.

doi:10.1016/j.biortech.2015.09.062

Title: Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

Journal Article · Sat Sep 26 00:00:00 EDT 2015 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2015.09.062· OSTI ID:1766805

Wang, Liang ^[1]; Ou, Mark S. ^[1]; Nieves, Ismael ^[1]; Erickson, John E. ^[1]; Vermerris, Wilfred ^[1]; Ingram, L. O. ^[1]; Shanmugam, K. T. ^[1]

Univ. of Florida, Gainesville, FL (United States)

In this study, a moderate thermophile Clostridium thermobutyricum is shown to ferment the sugars in sweet sorghum juice treated with invertase and supplemented with tryptone (10 g L^–1) and yeast extract (10 g L^–1) at 50 °C to 44 g L^–1 butyrate at a calculated highest volumetric productivity of 1.45 g L^–1 h^–1 (molar butyrate yield of 0.85 based on sugars fermented). This volumetric productivity is among the highest reported for batch fermentations. Sugars from acid and enzyme-treated sweet sorghum bagasse were also fermented to butyrate by this organism with a molar yield of 0.81 (based on the amount of cellulose and hemicellulose). By combining the results from juice and bagasse, the calculated yield of butyric acid is approximately 90 kg per tonne of fresh sweet sorghum stalk. Here, this study demonstrates that C. thermobutyricum can be an effective microbial biocatalyst for production of bio-based butyrate from renewable feedstocks at 50 °C.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1766805

Alternate ID(s):: OSTI ID: 1250354; OSTI ID: 1771308

Journal Information:: Bioresource Technology, Vol. 198, Issue 198; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 33 works

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Production of butyric acid from acid hydrolysate of corn husk in fermentation by Clostridium tyrobutyricum: kinetics and process economic analysis Xiao, Zhiping; Cheng, Chu; Bao, Teng Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1165-1	journal	June 2018
Microbial Production of Short Chain Fatty Acids from Lignocellulosic Biomass: Current Processes and Market Baumann, Ivan; Westermann, Peter BioMed Research International, Vol. 2016 https://doi.org/10.1155/2016/8469357	journal	January 2016
Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity Wang, Liang; Chauliac, Diane; Moritz, Brelan E. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1408-9	journal	March 2019
H-B-E (hexanol-butanol-ethanol) fermentation for the production of higher alcohols from syngas/waste gas Fernández-Naveira, Ánxela; Veiga, María C.; Kennes, Christian Journal of Chemical Technology & Biotechnology, Vol. 92, Issue 4 https://doi.org/10.1002/jctb.5194	journal	February 2017
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59 BASIC BIOLOGICAL SCIENCES
Butyrate
Fermentation
Clostridium thermobutyricum
Sweet sorghum juice
Sweet sorghum bagasse
Butyrate Fermentation Clostridium thermobutyricum Sweet sorghum juice Sweet sorghum bagasse

Title: Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

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