Engineering an increase in ethanol production by altering cofactor specificity

Lo, Jonathan; Guss, Adam M.; van Dijken, Johannes Pieter; Shaw, IV, Arthur J.; Olson, Daniel G.; Herring, Christopher D.; Argyros, D. Aaron; Caiazza, Nicky

Engineering an increase in ethanol production by altering cofactor specificity

Patent · Tue Sep 08 04:00:00 EDT 2020

OSTI ID:1735231

Lo, Jonathan; Guss, Adam M.; van Dijken, Johannes Pieter; Shaw, IV, Arthur J.; Olson, Daniel G.; Herring, Christopher D.; Argyros, D. Aaron; Caiazza, Nicky

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

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Research Organization:: Enchi Corp., Wellesley Hills, MA (United States); Dartmouth College, Hanover, NH (United States); UT-Battelle, LLC, Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: FC36-07GO17057

Assignee:: Enchi Corporation (Wellesley Hills, MA); Trustees of Dartmouth College (Hanover, NH); UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 10,767,196

Application Number:: 14/361,948

OSTI ID:: 1735231

Country of Publication:: United States

Language:: English

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