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Title: Engineering microorganisms to increase ethanol production by metabolic redirection

Patent ·
OSTI ID:1406573

The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Research Organization:
Enchi Corporation, Wellesley Hills, MA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-07GO17057; POS2-06ER64304
Assignee:
Enchi Corporation
Patent Number(s):
9,803,221
Application Number:
14/348,231
OSTI ID:
1406573
Resource Relation:
Patent File Date: 2012 Sep 28
Country of Publication:
United States
Language:
English

References (28)

Recycling system for manipulation of intracellular NADH availability patent August 2007
Recycling system for manipulation of intracellular NADH availability patent May 2010
High succinate producing bacteria patent-application April 2006
Native homoethanol Pathway for ethanol production in E. coli patent-application March 2009
Plasmids from Thermophilic Organisms, Vectors Derived Therefrom, and Uses Thereof patent-application March 2011
Product Recovery From Fermentation of Lignocellulosic Biomass patent-application July 2011
Construction of Protrophic/Celluloytic Yeast Strains Expressing Tethered and Secreted Cellulases patent-application August 2011
Positive And Negative Selectable Markers For Use In Thermophilic Organisms patent-application February 2013
Altered regulation of pyruvate kinase or co-overexpression of phosphofructokinase increases glycolytic fluxes in resting Escherichia coli journal March 2000
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for biotechnological production of organic acids and amino acids journal June 2006
Coordination of Adenosylmethionine to a Unique Iron Site of the [4Fe-4S] of Pyruvate Formate-Lyase Activating Enzyme:  A Mössbauer Spectroscopic Study
  • Krebs, Carsten; Broderick, William E.; Henshaw, Timothy F.
  • Journal of the American Chemical Society, Vol. 124, Issue 6, p. 912-913 https://doi.org/10.1021/ja017562i
journal February 2002
Protein tolerance to random amino acid change journal June 2004
Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. journal March 1988
Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli journal March 1998
Fermentation metabolism and its evolution in algae journal January 2013
High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes journal September 2011
Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase journal July 2002
Cellulase, Clostridia, and Ethanol journal March 2005
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum journal January 2013
A novel NADP+-dependent formate dehydrogenase from Burkholderia stabilis 15516: Screening, purification and characterization journal June 2010
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Deletion of the Cel48S cellulase from Clostridium thermocellum journal September 2010
Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production journal January 2010
Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria journal July 2006
The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications journal January 1987
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield journal September 2008
Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant journal August 2010
Atypical Glycolysis in Clostridium thermocellum journal February 2013