Engineered microorganisms capable of producing target compounds under anaerobic conditions

Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny; Hawkins, Andrew C; Urano, Jun; Bastian, Sabine; Arnold, Frances

Engineered microorganisms capable of producing target compounds under anaerobic conditions

Patent · Tue Jan 17 04:00:00 EST 2012

OSTI ID:1034914

Buelter, Thomas ^[1]; Meinhold, Peter ^[1]; Feldman, Reid M. Renny ^[2]; Hawkins, Andrew C ^[3]; Urano, Jun ^[4]; Bastian, Sabine ^[5]; Arnold, Frances ^[6]

Denver, CO
San Francisco, CA
Parker, CO
Irvine, CA
Pasadena, CA
La Canada, CA

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

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Research Organization:: Gevo, Inc. (Englewood, CO)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-07ER84893

Assignee:: Gevo, Inc. (Englewood, CO); California Institute of Technology (Pasadena, CA)

Patent Number(s):: 8,097,440

Application Number:: 13/269,937

OSTI ID:: 1034914

Country of Publication:: United States

Language:: English

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