Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

Wan, William; Clarke, Mairi; Norris, Michael J.; Kolesnikova, Larissa; Koehler, Alexander; Bornholdt, Zachary A.; Becker, Stephan; Saphire, Erica Ollmann; Briggs, John AG

doi:10.7554/elife.59225

Title: Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

Journal Article · Mon Oct 05 00:00:00 EDT 2020 · eLife

DOI:https://doi.org/10.7554/elife.59225· OSTI ID:1729696

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^[3]; Kolesnikova, Larissa ^[4]; Koehler, Alexander ^[4];

^[5]; Becker, Stephan ^[4];

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European Molecular Biology Lab. (EMBL), Heidelberg (Germany); Max Planck Society, Martinsried (Germany). Max Planck Inst. of Biogeochemistry
European Molecular Biology Lab. (EMBL), Heidelberg (Germany)
La Jolla Inst. for Immunology, La Jolla, CA (United States)
Phillips Univ., Marburg (Germany)
The Scripps Research Inst., La Jolla, CA (United States)
European Molecular Biology Lab. (EMBL), Heidelberg (Germany); MRC Lab. of Molecular Biology, Cambridge (United Kingdom)

Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE

OSTI ID:: 1729696

Journal Information:: eLife, Vol. 9, Issue 10; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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