OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions

Zhang, Jun; Gu, Mian; Liang, Ruisuhua; Shi, Xinyu; Chen, Lingling; Hu, Xu; Wang, Shichao; Dai, Xiaoli; Qu, Hongye; Li, Huanhuan; Xu, Guohua

doi:10.1111/nph.16931

Title: OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions

Journal Article · Sat Oct 10 00:00:00 EDT 2020 · New Phytologist

DOI:https://doi.org/10.1111/nph.16931· OSTI ID:1671837

Zhang, Jun ^[1];

^[2]; Liang, Ruisuhua ^[1]; Shi, Xinyu ^[1]; Chen, Lingling ^[1]; Hu, Xu ^[1]; Wang, Shichao ^[1]; Dai, Xiaoli ^[3]; Qu, Hongye ^[2]; Li, Huanhuan ^[1];

^[2]

State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China
State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilisation Nanjing 210095 China
State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China

Summary Plant Phosphate Transporter 1 (PHT1) proteins, probably the only influx transporters for phosphate (Pi) uptake, are partially degraded on sufficient Pi levels to prevent excessive Pi accumulation. Therefore, the basal/constitutive expression level of PHT1 genes is vital for maintaining Pi uptake under Pi‐replete conditions. Rice ( Oryza sativa ) OsPHT1;1 is a unique gene as it is highly expressed and not responsive to Pi, however the mechanism for maintaining its basal/constitutive expression remains unknown. Using biochemical and genetic approaches, we identified and functionally characterised the transcription factors maintaining the basal/constitutive expression of OsPHT1;1 . OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W‐box in the OsPHT1;1 promoter. Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1 . By contrast, oswrky21 oswrky108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi‐dependent manner. Moreover, similar to ospht1;1 mutants, plants expressing the OsWRKY21–SRDX fusion protein (a chimeric dominant suppressor) were impaired in Pi accumulation in Pi‐replete roots, accompanied by downregulation of OsPHT1;1 expression. Our findings demonstrated that rice WRKY transcription factors function redundantly to promote Pi uptake by activating OsPHT1;1 expression under Pi‐replete conditions, and represent a novel pathway independent of the central Pi signalling system.

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Sponsoring Organization:: USDOE

OSTI ID:: 1671837

Alternate ID(s):: OSTI ID: 1781900

Journal Information:: New Phytologist, Journal Name: New Phytologist Vol. 229 Journal Issue: 3; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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