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Title: Semi‐dominant mutation in the cysteine‐rich receptor‐like kinase gene, ALS 1 , conducts constitutive defence response in rice

Journal Article · · Plant Biology
DOI:https://doi.org/10.1111/plb.12896· OSTI ID:1472166
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  1. Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified Crops Academy of Agricultural Sciences Southwest University Chongqing 400715 China
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Abstract Plants have evolved a sophisticated two‐branch defence system to prevent the growth and spread of pathogen infection. The novel Cys‐rich repeat ( CRR ) containing receptor‐like kinases, known as CRK s, were reported to mediate defence resistance in plants. For rice, there are only two reports of CRK s. A semi‐dominant lesion mimic mutant als1 (apoptosis leaf and sheath 1) in rice was identified to demonstrate spontaneous lesions on the leaf blade and sheath. A map‐based cloning strategy was used for fine mapping and cloning of ALS 1 , which was confirmed to be a typical CRK in rice. Functional studies of ALS 1 were conducted, including phylogenetic analysis, expression analysis, subcellular location and blast resistance identification. Most pathogenesis‐related ( PR ) genes and other defence‐related genes were activated and up‐regulated to a high degree. ALS 1 was expressed mainly in the leaf blade and sheath, in which further study revealed that ALS 1 was present in the vascular bundles. ALS 1 was located in the cell membrane of rice protoplasts, and its mutation did not change its subcellular location. Jasmonic acid ( JA ) and salicylic acid ( SA ) accumulation were observed in als1 , and enhanced blast resistance was also observed. The mutation of ALS 1 caused a constitutively activated defence response in als1 . The results of our study imply that ALS 1 participates in a defence response resembling the common SA ‐, JA ‐ and NH 1 ‐mediated defence responses in rice.

Sponsoring Organization:
USDOE
OSTI ID:
1472166
Journal Information:
Plant Biology, Journal Name: Plant Biology Vol. 21 Journal Issue: 1; ISSN 1435-8603
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
Germany
Language:
English
Citation Metrics:
Cited by: 13 works
Citation information provided by
Web of Science

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