SWEET 11 and 15 as key players in seed filling in rice

Yang, Jungil; Luo, Dangping; Yang, Bing; Frommer, Wolf B.; Eom, Joon‐Seob

doi:10.1111/nph.15004

Title: SWEET 11 and 15 as key players in seed filling in rice

Journal Article · Fri Feb 02 00:00:00 EST 2018 · New Phytologist

DOI:https://doi.org/10.1111/nph.15004· OSTI ID:1419360

Yang, Jungil ^[1]; Luo, Dangping ^[2]; Yang, Bing ^[2]; Frommer, Wolf B. ^[3]; Eom, Joon‐Seob ^[1]

Institute for Molecular Physiology Heinrich‐Heine University Duesseldorf 40225 Duesseldorf Germany, Max Planck Institute for Plant Breeding Research 50829 Cologne Germany, Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA
Department of Genetics, Development, and Cell Biology Iowa State University Ames IA 50011 USA
Institute for Molecular Physiology Heinrich‐Heine University Duesseldorf 40225 Duesseldorf Germany, Max Planck Institute for Plant Breeding Research 50829 Cologne Germany, Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA, Institute of Transformative Bio‐Molecules (ITbM) Nagoya University Furo‐cho, Chikusa‐ku Nagoya Aichi 464‐8602 Japan

Summary Despite the relevance of seed‐filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. We hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis. We used a combination of mRNA quantification, histochemical analyses, translational promoter–reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling. In rice caryopses, SWEET 11 and 15 had the highest mRNA levels and proteins localized to four key sites: all regions of the nucellus at early stages; the nucellar projection close to the dorsal vein; the nucellar epidermis that surrounds the endosperm; and the aleurone. ossweet11 ; 15 double knockout lines accumulated starch in the pericarp, whereas caryopses did not contain a functional endosperm. Jointly, SWEET 11 and 15 show all the hallmarks of being necessary for seed filling with sucrose efflux functions at the nucellar projection and a role in transfer across the nucellar epidermis/aleurone interface, delineating two major steps for apoplasmic seed filling, observations that are discussed in relation to observations made in rice and barley regarding the relative prevalence of these two potential import routes.

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Research Organization:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: DE‐FG02‐04ER15542; FG02-04ER15542

OSTI ID:: 1419360

Alternate ID(s):: OSTI ID: 1419361; OSTI ID: 1500104

Journal Information:: New Phytologist, Journal Name: New Phytologist Vol. 218 Journal Issue: 2; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 145 works

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