Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays

Bezrutczyk, Margaret; Hartwig, Thomas; Horschman, Marc; Char, Si Nian; Yang, Jinliang; Yang, Bing; Frommer, Wolf B.; Sosso, Davide

doi:10.1111/nph.15021

Title: Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays

Journal Article · Fri Feb 16 00:00:00 EST 2018 · New Phytologist

DOI:https://doi.org/10.1111/nph.15021· OSTI ID:1421567

Bezrutczyk, Margaret ^[1]; Hartwig, Thomas ^[1]; Horschman, Marc ^[2]; Char, Si Nian ^[3]; Yang, Jinliang ^[4]; Yang, Bing ^[3]; Frommer, Wolf B. ^[1]; Sosso, Davide ^[2]

Institute for Molecular Physiology Heinrich Heine University Düsseldorf Düsseldorf 40225 Germany, Max Planck Institute for Plant Breeding Research Cologne 50829 Germany, Department of Plant Biology Carnegie Science 260 Panama St Stanford CA 94305 USA
Department of Plant Biology Carnegie Science 260 Panama St Stanford CA 94305 USA
Department of Genetics, Development, and Cell Biology Iowa State University Ames IA 50011 USA
Department of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE 68588 USA

Summary Crop yield depends on efficient allocation of sucrose from leaves to seeds. In Arabidopsis, phloem loading is mediated by a combination of SWEET sucrose effluxers and subsequent uptake by SUT 1/ SUC 2 sucrose/H ⁺ symporters. Zm SUT 1 is essential for carbon allocation in maize, but the relative contribution to apoplasmic phloem loading and retrieval of sucrose leaking from the translocation path is not known. Here we analysed the contribution of SWEET s to phloem loading in maize. We identified three leaf‐expressed SWEET sucrose transporters as key components of apoplasmic phloem loading in Zea mays L. Zm SWEET 13 paralogues (a, b, c) are among the most highly expressed genes in the leaf vasculature. Genome‐edited triple knock‐out mutants were severely stunted. Photosynthesis of mutants was impaired and leaves accumulated high levels of soluble sugars and starch. RNA ‐seq revealed profound transcriptional deregulation of genes associated with photosynthesis and carbohydrate metabolism. Genome‐wide association study (GWAS) analyses may indicate that variability in Zm SWEET 13s correlates with agronomical traits, especifically flowering time and leaf angle. This work provides support for cooperation of three Zm SWEET 13s with Zm SUT 1 in phloem loading in Z. mays .

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Research Organization:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: DE‐FG02‐04ER15542; FG02-04ER15542

OSTI ID:: 1421567

Alternate ID(s):: OSTI ID: 1421568; OSTI ID: 1500215

Journal Information:: New Phytologist, Journal Name: New Phytologist Vol. 218 Journal Issue: 2; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 96 works

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