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The fine structure of the nucleolus during mitosis in the grasshopper neuroblast cell

Journal Article · · Journal of Cell Biology
DOI:https://doi.org/10.1083/jcb.24.3.349· OSTI ID:1629499
 [1]
  1. Cornell Univ., Ithaca, NY (United States). Dept. of Anatomy; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
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The behavior of the nucleolus during mitosis was studied by electron microscopy in neuroblast cells of the grasshopper embryo, Chortophaga viridifasciata. Living neuroblast cells were observed in the light microscope, and their mitotic stages were identified and recorded. The cells were fixed and embedded; alternate thick and thin sections were made for light and electron microscopy. The interphase nucleolus consists of two fine structural components arranged in separate zones. Concentrations of 150 A granules form a dense peripheral zone, while the central regions are composed of a homogeneous background substance. Observations show that nucleolar dissolution in prophase occurs in two steps with a preliminary loss of the background substance followed by a dispersal of the granules. Nucleolar material reappears at anaphase as small clumps or layers at the chromosome surfaces. These later form into definite bodies, which disappear as the nucleolus grows in telophase. Evidence suggests both a collecting and a synthesizing role for the nucleolus-associated chromatin. The final, mature nucleolar form is produced by a rearrangement of the fine structural components and an increase in their mass.
Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
US Atomic Energy Commission (AEC); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1629499
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 3 Vol. 24; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

References (1)

Specific chromosomal control of the nucleolus and of the cytoplasm in wheat journal August 1960

Cited By (8)

Nucleolus: the fascinating nuclear body journal November 2007
Nucleolar Assembly of the Rrna Processing Machinery in Living Cells journal May 2001
Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription journal August 2000
A correlated light- and electronmicroscope investigation of the structural evolution of the nucleolus during the cell cycle in plant meristematic cells (Allium Porrum) journal October 1974
Morphological Studies of Nucleologenesis in G iardia lamblia: Nucleologenesis in Giardia
  • Lara-Martínez, Reyna; De Lourdes Segura-Valdez, María; De La Mora-De La Mora, Ignacio
  • The Anatomical Record, Vol. 299, Issue 5 https://doi.org/10.1002/ar.23323
journal February 2016
Nucleologenesis in Trypanosoma cruzi journal April 2016
A ratiometric optical imaging probe for intracellular pH based on modulation of europium emission journal January 2008
Assembly and disassembly of the nucleolus during the cell cycle journal May 2011

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59 BASIC BIOLOGICAL SCIENCES