Mitotic effects of monochromatic ultraviolet radiation at 225, 265, and 280 nm on eleven stages of the cell cycle of the grasshopper neuroblast in culture. II. Changes in progression rate and cell sequence between the stage irradiated and nuclear membrane breakdown

Journal Article · Fri Oct 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574535· OSTI ID:7342687

Carlson, J G

Portions of embryos of the grasshopper, Chortophaga viridifasciata (DeGeer), were cultured in hanging drops under quartz cover slips. Immediately after exposure to 225, 265, or 280 nm radiation, microscope observations at 38/sup 0/C were begun. The morphologically identified stage and the time after treatment of selected neuroblasts were recorded at short-time intervals until prometaphase was reached. Mitotic retardation induced by irradiation of prereplication stages (metaphase, anaphase, or early telophase) or S phase (middle or late telophase, interphase, or very early prophase) is greatest in postreplication stages (early, middle, and late prophase) and absent or minimal in stages morphologically identified as parts of S phase. Ultraviolet irradiation superimposes on the normal diversity of progression rates an additional variation factor, so that cells do not necessarily reach prometaphase in the order of their sequence at the time of treatment. This suggests the need for caution in ascribing particular radiosensitivities to substages of limited duration on the basis of the order in which they attain a subsequent stage.

• Research Organization: Univ. of Tennessee, Knoxville
• OSTI ID: 7342687
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 68:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560154* -- Radiation Effects on Animals-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
ELECTROMAGNETIC RADIATION
EMBRYOS
GRASSHOPPERS
INSECTS
INVERTEBRATES
IRRADIATION
NERVE CELLS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
SOMATIC CELLS
ULTRAVIOLET RADIATION