Strandedness of Vicia faba chromosomes as revealed by enzyme digestion studies
Journal Article
·
· Journal of Cell Biology
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division
Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- Grant/Contract Number:
- AC05-00OR22725
- OSTI ID:
- 1629443
- Journal Information:
- Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 26; ISSN 0021-9525
- Publisher:
- Rockefeller University PressCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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