Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

Bordowitz, Juliana R.; Montgomery, Beronda L.

doi:10.3390/s100706969

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

Journal Article · Thu Jul 01 00:00:00 EDT 2010 · Sensors

DOI:https://doi.org/10.3390/s100706969· OSTI ID:1628532

Bordowitz, Juliana R. ^[1]; Montgomery, Beronda L. ^[2]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Cell and Molecular Biology Program; Univ. of California, San Diego, La Jolla, CA (United States). Division of Biological Sciences; DOE/OSTI
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Cell and Molecular Biology Program; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology

Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1628532

Journal Information:: Sensors, Journal Name: Sensors Journal Issue: 7 Vol. 10; ISSN SENSC9; ISSN 1424-8220

Publisher:: MDPI AGCopyright Statement

Country of Publication:: United States

Language:: English

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Cited By (4)

Light Quantity Affects the Regulation of Cell Shape in Fremyella diplosiphon Pattanaik, Bagmi; Whitaker, Melissa J.; Montgomery, Beronda L. Frontiers in Microbiology, Vol. 3 https://doi.org/10.3389/fmicb.2012.00170	journal	January 2012
Regulation of BolA abundance mediates morphogenesis in Fremyella diplosiphon Singh, Shailendra P.; Montgomery, Beronda L. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01215	journal	November 2015
The Tryptophan-Rich Sensory Protein (TSPO) is Involved in Stress-Related and Light-Dependent Processes in the Cyanobacterium Fremyella diplosiphon Busch, Andrea W. U.; Montgomery, Beronda L. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01393	journal	December 2015
Complementary Assessment of Commercial Photoluminescent Pigments Printed on Cotton Fabric Gorgieva, Selestina; Virant, Natalija; Ojstršek, Alenka Polymers, Vol. 11, Issue 7 https://doi.org/10.3390/polym11071216	journal	July 2019

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
42 ENGINEERING
Chemistry
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confocal laser scanning microscopy
cyanobacteria
light
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Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

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