Regulation of BolA abundance mediates morphogenesis in Fremyella diplosiphon

Singh, Shailendra P.; Montgomery, Beronda L.

doi:10.3389/fmicb.2015.01215

Regulation of BolA abundance mediates morphogenesis in Fremyella diplosiphon

Journal Article · Thu Nov 05 00:00:00 EST 2015 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2015.01215· OSTI ID:1242038

Singh, Shailendra P. ^[1]; Montgomery, Beronda L. ^[2]

Michigan State Univ., East Lansing, MI (United States); Michigan State University
Michigan State Univ., East Lansing, MI (United States)

Filamentous cyanobacterium Fremyella diplosiphon is known to alter its pigmentation and morphology during complementary chromatic acclimation (CCA) to efficiently harvest available radiant energy for photosynthesis. F. diplosiphon cells are rectangular and filaments are longer under green light (GL), whereas smaller, spherical cells and short filaments are prevalent under red light (RL). Light regulation of bolA morphogene expression is correlated with photoregulation of cellular morphology in F. diplosiphon. Here, we investigate a role for quantitative regulation of cellular BolA protein levels in morphology determination. Overexpression of bolA in WT was associated with induction of RL-characteristic spherical morphology even when cultures were grown under GL. Overexpression of bolA in a ΔrcaE background, which lacks cyanobacteriochrome photosensor RcaE and accumulates lower levels of BolA than WT, partially reverted the cellular morphology of the strain to a WT-like state. Overexpression of BolA in WT and ΔrcaE backgrounds was associated with decreased cellular reactive oxygen species (ROS) levels and an increase in filament length under both GL and RL. Morphological defects and high ROS levels commonly observed in ΔrcaE could, thus, be in part due to low accumulation of BolA. Together, these findings support an emerging model for RcaE-dependent photoregulation of BolA in controlling the cellular morphology of F. diplosiphon during CCA.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1242038

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 6; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
BolA
cellularmorphology
complementary chromatic acclimation(CCA)
cyanobacteria
light signaling
photomorphogenesis

Regulation of BolA abundance mediates morphogenesis in Fremyella diplosiphon

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References (61)

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