Morphogenes bolA and mreB mediate the photoregulation of cellular morphology during complementary chromatic acclimation in Fremyella diplosiphon: Photoregulation of morphogenes mediates cell shape

Singh, Shailendra P.; Montgomery, Beronda L.

doi:10.1111/mmi.12649

Morphogenes bolA and mreB mediate the photoregulation of cellular morphology during complementary chromatic acclimation in Fremyella diplosiphon: Photoregulation of morphogenes mediates cell shape

Journal Article · Thu May 29 04:00:00 EDT 2014 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.12649· OSTI ID:1603483

Singh, Shailendra P. ^[1]; Montgomery, Beronda L. ^[2]

Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.; MSU DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.

Photoregulation of pigmentation during complementary chromatic acclimation (CCA) is well studied in Fremyella diplosiphon; however, mechanistic insights into the CCA-associated morphological changes are still emerging. F. diplosiphon cells are rectangular under green light (GL), whereas cells are smaller and spherical under red light (RL). Here, we investigate the role of morphogenes bolA and mreB during CCA using gene expression and gene function analyses. The F. diplosiphon bolA gene is essential as its complete removal from the genome was unsuccessful. Depletion of bolA resulted in slow growth, morphological defects and the accumulation of high levels of reactive oxygen species in a partially segregated ΔbolA strain. Higher expression of bolA was observed under RL and was correlated with lower expression of mreB and mreC genes in wild type. In a ΔrcaE strain that lacks the red-/green-responsive RcaE photoreceptor, the expression of bolA and mre genes was altered under both RL and GL. Observed gene expression relationships suggest that mreB and mreC expression is controlled by RcaE-dependent photoregulation of bolA expression. Expression of F. diplosiphon bolA and mreB homologues in Escherichia coli demonstrated functional conservation of the encoded proteins. Together, these studies establish roles for bolA and mreB in RcaE-dependent regulation of cellular morphology.

Research Organization:: Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Science Foundation (NSF)

DOE Contract Number:: FG02-91ER20021

OSTI ID:: 1603483

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 1 Vol. 93; ISSN 0950-382X

Publisher:: Wiley

Country of Publication:: United States

Language:: English

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