Stress response to CO2 deprivation by Arabidopsis thaliana in plant cultures

Banerjee, Souvik; Siemianowski, Oskar; Liu, Meiling; Lind, Kara R.; Tian, Xinchun; Nettleton, Dan; Cademartiri, Ludovico

doi:10.1371/journal.pone.0212462

Stress response to CO₂ deprivation by Arabidopsis thaliana in plant cultures

Journal Article · Wed Mar 13 00:00:00 EDT 2019 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0212462· OSTI ID:1627878

Banerjee, Souvik ^[1]; Siemianowski, Oskar ^[2]; Liu, Meiling ^[3]; Lind, Kara R. ^[2]; Tian, Xinchun ^[2]; Nettleton, Dan ^[4]; ^[5]

Iowa State Univ., Ames, IA (United States). Dept. of Materials Science & Engineering; DOE/OSTI
Iowa State Univ., Ames, IA (United States). Dept. of Materials Science & Engineering
Iowa State Univ. of Science and Technology, Ames, IA (United States). Dept. of Statistics; Iowa State Univ. of Science and Technology, Ames, IA (United States). Bioinformatics and Computational Biology Program
Iowa State Univ. of Science and Technology, Ames, IA (United States). Dept. of Statistics
Iowa State Univ., Ames, IA (United States). Dept. of Materials Science & Engineering; Iowa State Univ. of Science and Technology, Ames, IA (United States). Dept. of Chemical & Biological Engineering; Ames Lab., Ames, IA (United States)

After being the standard plant propagation protocol for decades, cultures of Arabidopsis thaliana sealed with Parafilm remain common today out of practicality, habit, or necessity (as in co-cultures with microorganisms). Regardless of concerns over the aeration of these cultures, no investigation has explored the CO₂ transport inside these cultures and its effect on the plants. Thereby, it was impossible to assess whether Parafilm-seals used today or in thousands of older papers in the literature constitute a treatment, and whether this treatment could potentially affect the study of other treatments.For the first time we report the CO₂ concentrations in Parafilm-sealed cultures of A. thaliana with a 1 minute temporal resolution, and the transcriptome comparison with aerated cultures. The data show significant CO₂ deprivation to the plants, a drastic suppression of photosynthesis, respiration, starch accumulation, chlorophyll biosynthesis, and an increased accumulation of reactive oxygen species. Most importantly, CO₂ deprivation occurs as soon as the cotyledons emerge. Gene expression analysis indicates a significant alteration of 35% of the pathways when compared to aerated cultures, especially in stress response and secondary metabolism processes. On the other hand, the observed increase in the production of glucosinolates and flavonoids suggests intriguing possibilities for CO₂ deprivation as an organic biofortification treatment in high-value crops.

View Accepted Manuscript (DOE)

Research Organization:: Ames Lab., Ames, IA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1627878

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 3 Vol. 14; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Wrapping culture plates with Parafilm M® increases Caenorhabditis elegans growth Shinn-Thomas, Jessica; Scanga, Sara E.; Spica, Patrick S. TAGC 2020 https://doi.org/10.6084/m9.figshare.12133467.v1	image	January 2020
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