Clostridium botulinum Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and bont/F5

Dover, Nir; Barash, Jason R.; Hill, Karen K.; Davenport, Karen W.; Teshima, Hazuki; Xie, Gary; Arnon, Stephen S.

doi:10.1371/journal.pone.0061205

***Clostridium botulinum* Strain Af84 Contains Three Neurotoxin Gene Clusters: Bont/A2, bont/F4 and *bont/F5***

Journal Article · Fri Apr 12 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0061205· OSTI ID:1627599

Dover, Nir ^[1]; Barash, Jason R. ^[2]; Hill, Karen K. ^[3]; Davenport, Karen W. ^[3]; Teshima, Hazuki ^[3]; Xie, Gary ^[3]; Arnon, Stephen S. ^[2]

California Department of Public Health, Richmond, CA (United States); DOE/OSTI
California Department of Public Health, Richmond, CA (United States)
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: California Department of Public Health; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1627599

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 4 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (43)

Hemoperitoneum From Spontaneous Rupture of Neonatal Neuroblastoma Brock, Carol E. Archives of Pediatrics & Adolescent Medicine, Vol. 136, Issue 4 https://doi.org/10.1001/archpedi.1982.03970400088026	journal	April 1982
Infant Botulism: Three Cases in a Small Town Istre, Gregory R. American Journal of Diseases of Children, Vol. 140, Issue 10 https://doi.org/10.1001/archpedi.1986.02140240059027	journal	October 1986
Botulinum Toxin as a Biological Weapon: Medical and Public Health Management Arnon, Stephen S.; Schechter, Robert; Inglesby, Thomas V. JAMA, Vol. 285, Issue 8 https://doi.org/10.1001/jama.285.8.1059	journal	February 2001
Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes Marshall, Kristin M.; Bradshaw, Marite; Pellett, Sabine Biochemical and Biophysical Research Communications, Vol. 361, Issue 1 https://doi.org/10.1016/j.bbrc.2007.06.166	journal	September 2007
Genome sequencing in microfabricated high-density picolitre reactors Margulies, Marcel; Egholm, Michael; Altman, William E. Nature, Vol. 437, Issue 7057, p. 376-380 https://doi.org/10.1038/nature03959	journal	July 2005
ACT: the Artemis comparison tool Carver, T. J.; Rutherford, K. M.; Berriman, M. Bioinformatics, Vol. 21, Issue 16 https://doi.org/10.1093/bioinformatics/bti553	journal	June 2005
Fast algorithms for large-scale genome alignment and comparison Delcher, A. L. Nucleic Acids Research, Vol. 30, Issue 11 https://doi.org/10.1093/nar/30.11.2478	journal	June 2002
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs Zerbino, D. R.; Birney, E. Genome Research, Vol. 18, Issue 5 https://doi.org/10.1101/gr.074492.107	journal	February 2008
Base-Calling of Automated Sequencer Traces Using Phred. I. Accuracy Assessment Ewing, Brent; Hillier, LaDeana; Wendl, Michael C. Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.175	journal	March 1998
Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities Ewing, Brent; Green, Phil Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.186	journal	March 1998
Consed: A Graphical Tool for Sequence Finishing Gordon, David; Abajian, Chris; Green, Phil Genome Research, Vol. 8, Issue 3 https://doi.org/10.1101/gr.8.3.195	journal	March 1998
Novel Structural Elements within the Nonproteolytic Clostridium botulinum Type F Toxin Gene Cluster Dover, N.; Barash, J. R.; Hill, K. K. Applied and Environmental Microbiology, Vol. 77, Issue 5 https://doi.org/10.1128/aem.02422-10	journal	December 2010
Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes Jacobson, Mark J.; Lin, Guangyun; Raphael, Brian Applied and Environmental Microbiology, Vol. 74, Issue 9 https://doi.org/10.1128/aem.02828-07	journal	March 2008
Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F Raphael, Brian H.; Choudoir, Mallory J.; Lúquez, Carolina Applied and Environmental Microbiology, Vol. 76, Issue 14 https://doi.org/10.1128/aem.03109-09	journal	May 2010
Genetic Diversity among Botulinum Neurotoxin-Producing Clostridial Strains Hill, K. K.; Smith, T. J.; Helma, C. H. Journal of Bacteriology, Vol. 189, Issue 3 https://doi.org/10.1128/jb.01180-06	journal	November 2006
Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism. Hall, J. D.; McCroskey, L. M.; Pincomb, B. J. Journal of Clinical Microbiology, Vol. 21, Issue 4 https://doi.org/10.1128/jcm.21.4.654-655.1985	journal	January 1985
Examination of feces and serum for diagnosis of infant botulism in 336 patients. Hatheway, C. L.; McCroskey, L. M. Journal of Clinical Microbiology, Vol. 25, Issue 12 https://doi.org/10.1128/jcm.25.12.2334-2338.1987	journal	January 1987
Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpressed type B toxin genes in type A toxigenic organisms. Franciosa, G.; Ferreira, J. L.; Hatheway, C. L. Journal of Clinical Microbiology, Vol. 32, Issue 8 https://doi.org/10.1128/jcm.32.8.1911-1917.1994	journal	January 1994
First Case of Infant Botulism Caused by Clostridium baratii Type F in California Barash, J. R.; Tang, T. W. H.; Arnon, S. S. Journal of Clinical Microbiology, Vol. 43, Issue 8 https://doi.org/10.1128/jcm.43.8.4280-4282.2005	journal	August 2005
Infantile botulism. Smith, G. E.; Hinde, F.; Westmoreland, D. Archives of Disease in Childhood, Vol. 64, Issue 6 https://doi.org/10.1136/adc.64.6.871	journal	June 1989
Evidence that Plasmid-Borne Botulinum Neurotoxin Type B Genes Are Widespread among Clostridium botulinum Serotype B Strains Franciosa, Giovanna; Maugliani, Antonella; Scalfaro, Concetta PLoS ONE, Vol. 4, Issue 3 https://doi.org/10.1371/journal.pone.0004829	journal	March 2009
Solexa Ltd Bennett, Simon Pharmacogenomics, Vol. 5, Issue 4 https://doi.org/10.1517/14622416.5.4.433	journal	June 2004
Architecture of the type II secretion and type IV pilus machineries Ayers, Melissa; Howell, P. Lynne; Burrows, Lori L. Future Microbiology, Vol. 5, Issue 8 https://doi.org/10.2217/fmb.10.76	journal	August 2010
A 3-Day-Old Boy with Acute Flaccid Paralysis Al-Sayyed, Ban Pediatric Annals, Vol. 38, Issue 9 https://doi.org/10.3928/00904481-20090820-01	journal	September 2009
Detection And Identification of Clostridium botulinum Neurotoxins Hatheway, Charles L.; Ferreira, Joseph L. Advances in Experimental Medicine and Biology https://doi.org/10.1007/978-1-4613-0361-9_39	book	January 1996
Infant botulism, type F, presenting at 54 hours of life Keet, Corinne A.; Fox, Christine K.; Margeta, Marta Pediatric Neurology, Vol. 32, Issue 3 https://doi.org/10.1016/j.pediatrneurol.2004.09.003	journal	March 2005
MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods Tamura, K.; Peterson, D.; Peterson, N. Molecular Biology and Evolution, Vol. 28, Issue 10 https://doi.org/10.1093/molbev/msr121	journal	May 2011
A Second case of Infant Botulism type f Caused by Clostridium Baratii Walsh, Joanna E.; Abinun, Mario; Malik Peiris, J. S. The Pediatric Infectious Disease Journal, Vol. 14, Issue 10 https://doi.org/10.1097/00006454-199510000-00022	journal	January 1995
Neurotoxin Gene Clusters in Clostridium botulinum Type Ab Strains Lúquez, Carolina; Raphael, Brian H.; Maslanka, Susan E. Applied and Environmental Microbiology, Vol. 75, Issue 19 https://doi.org/10.1128/AEM.01009-09	journal	August 2009
Novel Structural Elements within the Nonproteolytic Clostridium botulinum Type F Toxin Gene Cluster Dover, N.; Barash, J. R.; Hill, K. K. Applied and Environmental Microbiology, Vol. 77, Issue 5 https://doi.org/10.1128/AEM.02422-10	journal	December 2010
Analysis of Neurotoxin Cluster Genes in Clostridium botulinum Strains Producing Botulinum Neurotoxin Serotype A Subtypes Jacobson, Mark J.; Lin, Guangyun; Raphael, Brian Applied and Environmental Microbiology, Vol. 74, Issue 9 https://doi.org/10.1128/AEM.02828-07	journal	March 2008
Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F Raphael, Brian H.; Choudoir, Mallory J.; Lúquez, Carolina Applied and Environmental Microbiology, Vol. 76, Issue 14 https://doi.org/10.1128/AEM.03109-09	journal	May 2010
Genetic Diversity among Botulinum Neurotoxin-Producing Clostridial Strains Hill, K. K.; Smith, T. J.; Helma, C. H. Journal of Bacteriology, Vol. 189, Issue 3 https://doi.org/10.1128/JB.01180-06	journal	November 2006
Identical Novel A5(B3') Botulinum Neurotoxin Gene Arrangements Isolated from Widely Disparate Geographical and Patient Sources Suggest Their Independent Origins Dover, N.; Barash, J. R.; Arnon, S. S. Journal of Clinical Microbiology, Vol. 48, Issue 5 https://doi.org/10.1128/JCM.00468-10	journal	April 2010
Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism. Hall, J. D.; McCroskey, L. M.; Pincomb, B. J. Journal of Clinical Microbiology, Vol. 21, Issue 4 https://doi.org/10.1128/JCM.21.4.654-655.1985	journal	January 1985
Examination of feces and serum for diagnosis of infant botulism in 336 patients. Hatheway, C. L.; McCroskey, L. M. Journal of Clinical Microbiology, Vol. 25, Issue 12 https://doi.org/10.1128/JCM.25.12.2334-2338.1987	journal	January 1987
Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpressed type B toxin genes in type A toxigenic organisms. Franciosa, G.; Ferreira, J. L.; Hatheway, C. L. Journal of Clinical Microbiology, Vol. 32, Issue 8 https://doi.org/10.1128/JCM.32.8.1911-1917.1994	journal	January 1994
Dual Toxin-Producing Strain of Clostridium botulinum Type Bf Isolated from a California Patient with Infant Botulism Barash, J. R.; Arnon, S. S. Journal of Clinical Microbiology, Vol. 42, Issue 4 https://doi.org/10.1128/JCM.42.4.1713-1715.2004	journal	April 2004
First Case of Infant Botulism Caused by Clostridium baratii Type F in California Barash, J. R.; Tang, T. W. H.; Arnon, S. S. Journal of Clinical Microbiology, Vol. 43, Issue 8 https://doi.org/10.1128/JCM.43.8.4280-4282.2005	journal	August 2005
The RAST Server: Rapid Annotations using Subsystems Technology Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A. BMC Genomics, Vol. 9, Issue 1, Article No. 75 https://doi.org/10.1186/1471-2164-9-75	journal	January 2008
Recombination and insertion events involving the botulinum neurotoxin complex genes in Clostridium botulinum types A, B, E and F and Clostridium butyricumtype E strains Hill, Karen K.; Xie, Gary; Foley, Brian T. BMC Biology, Vol. 7, Issue 1 https://doi.org/10.1186/1741-7007-7-66	journal	October 2009
Versatile and open software for comparing large genomes Kurtz, Stefan; Phillippy, Adam; Delcher, Arthur L. Genome Biology, Vol. 5, Issue 2, R12 https://doi.org/10.1186/gb-2004-5-2-r12	journal	January 2004
Analysis of the Neurotoxin Complex Genes in Clostridium botulinum A1-A4 and B1 Strains: BoNT/A3, /Ba4 and /B1 Clusters Are Located within Plasmids Smith, Theresa J.; Hill, Karen K.; Foley, Brian T. PLoS ONE, Vol. 2, Issue 12 https://doi.org/10.1371/journal.pone.0001271	journal	December 2007

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High Sensitivity Detection of Active Botulinum Neurotoxin by Glyco-Quantitative Polymerase Chain-Reaction Kwon, Seok Joon; Jeong, Eun Ji; Yoo, Yung Choon Analytical Chemistry, Vol. 86, Issue 5 https://doi.org/10.1021/ac500262d	journal	February 2014
Three Classes of Plasmid (47–63 kb) Carry the Type B Neurotoxin Gene Cluster of Group II Clostridium botulinum Carter, Andrew T.; Austin, John W.; Weedmark, Kelly A. Genome Biology and Evolution, Vol. 6, Issue 8 https://doi.org/10.1093/gbe/evu164	journal	July 2014
Evolution of Chromosomal Clostridium botulinum Type E Neurotoxin Gene Clusters: Evidence Provided by Their Rare Plasmid-Borne Counterparts Carter, Andrew T.; Austin, John W.; Weedmark, Kelly A. Genome Biology and Evolution, Vol. 8, Issue 3 https://doi.org/10.1093/gbe/evw017	journal	March 2016
Purification and Characterization of Botulinum Neurotoxin FA from a Genetically Modified Clostridium botulinum Strain Pellett, Sabine; Tepp, William H.; Bradshaw, Marite mSphere, Vol. 1, Issue 1 https://doi.org/10.1128/msphere.00100-15	journal	February 2016
Comparative genomic analyses reveal broad diversity in botulinum-toxin-producing Clostridia Williamson, Charles H. D.; Sahl, Jason W.; Smith, Theresa J. BMC Genomics, Vol. 17, Issue 1 https://doi.org/10.1186/s12864-016-2502-z	journal	March 2016
Isolation and Functional Characterization of the Novel Clostridium botulinum Neurotoxin A8 Subtype Kull, Skadi; Schulz, K. Melanie; Strotmeier, Jasmin Weisemann née PLOS ONE, Vol. 10, Issue 2 https://doi.org/10.1371/journal.pone.0116381	journal	February 2015
Clostridium botulinum Genomes and Genetic Diversity Smith, Theresa J. Molecular Aspects of Botulinum Neurotoxin https://doi.org/10.1007/978-1-4614-9454-6_10	book	January 2014
Identification and characterization of a novel botulinum neurotoxin Zhang, Sicai; Masuyer, Geoffrey; Zhang, Jie Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14130	journal	August 2017
Botulinum neurotoxins: genetic, structural and mechanistic insights Rossetto, Ornella; Pirazzini, Marco; Montecucco, Cesare Nature Reviews Microbiology, Vol. 12, Issue 8 https://doi.org/10.1038/nrmicro3295	journal	June 2014
A Novel Strain of Clostridium botulinum That Produces Type B and Type H Botulinum Toxins Barash, Jason R.; Arnon, Stephen S. The Journal of Infectious Diseases, Vol. 209, Issue 2 https://doi.org/10.1093/infdis/jit449	journal	October 2013
Molecular Characterization of a Novel Botulinum Neurotoxin Type H Gene Dover, Nir; Barash, Jason R.; Hill, Karen K. The Journal of Infectious Diseases, Vol. 209, Issue 2 https://doi.org/10.1093/infdis/jit450	journal	October 2013
Genetic Characterization and Comparison of Clostridium botulinum Isolates from Botulism Cases in Japan between 2006 and 2011 Kenri, Tsuyoshi; Sekizuka, Tsuyoshi; Yamamoto, Akihiko Applied and Environmental Microbiology, Vol. 80, Issue 22 https://doi.org/10.1128/aem.02134-14	journal	September 2014

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59 BASIC BIOLOGICAL SCIENCES
Clostridium botulinum
antitoxins
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genomics
neurotoxins
nucleotide sequencing
polymerase chain reactions
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