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Title: Genetic diversity within the botulinum neurotoxin-producing bacteria and their neurotoxins

Abstract

The recent availability of multiple Clostridium botulinum genomic sequences has initiated a new genomics era that strengthens our understanding of the bacterial species that produce botulinum neurotoxins (BoNTs). Analysis of the genomes has reinforced the historical Group I–VI designations and provided evidence that the bont genes can be located within the chromosome, phage or plasmids. The sequences provide the opportunity to examine closely the variation among the toxin genes, the composition and organization of the toxin complex, the regions flanking the toxin complex and the location of the toxin within different bacterial strains. These comparisons provide evidence of horizontal gene transfer and site-specific insertion and recombination events that have contributed to the variation observed among the neurotoxins. In this work, examples that have contributed to the variation observed in serotypes A-H strains are presented to illustrate the mechanisms that have contributed to their variation.

Authors:
 [1]; ORCiD logo [1]; ORCiD logo [1];  [2]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. US Army Medical Research Inst. of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States). Molecular and Translational Sciences Division
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
OSTI Identifier:
1457238
Report Number(s):
LA-UR-15-23061
Journal ID: ISSN 0041-0101
Grant/Contract Number:  
AC52-06NA25396; U01 AI056493
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Toxicon
Additional Journal Information:
Journal Volume: 107; Journal Issue: PA; Journal ID: ISSN 0041-0101
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; genetic diversity; botulinum neurotoxin; Clostridium botulinum

Citation Formats

Hill, Karen Koons, Xie, Gary, Foley, Brian T., and Smith, Theresa J. Genetic diversity within the botulinum neurotoxin-producing bacteria and their neurotoxins. United States: N. p., 2015. Web. doi:10.1016/j.toxicon.2015.09.011.
Hill, Karen Koons, Xie, Gary, Foley, Brian T., & Smith, Theresa J. Genetic diversity within the botulinum neurotoxin-producing bacteria and their neurotoxins. United States. doi:10.1016/j.toxicon.2015.09.011.
Hill, Karen Koons, Xie, Gary, Foley, Brian T., and Smith, Theresa J. Fri . "Genetic diversity within the botulinum neurotoxin-producing bacteria and their neurotoxins". United States. doi:10.1016/j.toxicon.2015.09.011. https://www.osti.gov/servlets/purl/1457238.
@article{osti_1457238,
title = {Genetic diversity within the botulinum neurotoxin-producing bacteria and their neurotoxins},
author = {Hill, Karen Koons and Xie, Gary and Foley, Brian T. and Smith, Theresa J.},
abstractNote = {The recent availability of multiple Clostridium botulinum genomic sequences has initiated a new genomics era that strengthens our understanding of the bacterial species that produce botulinum neurotoxins (BoNTs). Analysis of the genomes has reinforced the historical Group I–VI designations and provided evidence that the bont genes can be located within the chromosome, phage or plasmids. The sequences provide the opportunity to examine closely the variation among the toxin genes, the composition and organization of the toxin complex, the regions flanking the toxin complex and the location of the toxin within different bacterial strains. These comparisons provide evidence of horizontal gene transfer and site-specific insertion and recombination events that have contributed to the variation observed among the neurotoxins. In this work, examples that have contributed to the variation observed in serotypes A-H strains are presented to illustrate the mechanisms that have contributed to their variation.},
doi = {10.1016/j.toxicon.2015.09.011},
journal = {Toxicon},
issn = {0041-0101},
number = PA,
volume = 107,
place = {United States},
year = {2015},
month = {9}
}

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Cited by: 35 works
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Works referencing / citing this record:

The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes
journal, March 2018


A Rapid, Sensitive, and Portable Biosensor Assay for the Detection of Botulinum Neurotoxin Serotype A in Complex Food Matrices
journal, November 2018


Critical Analysis of Neuronal Cell and the Mouse Bioassay for Detection of Botulinum Neurotoxins
journal, December 2019